FCM (Flow Cytometry)
(Overlay histogram showing 293F transfected cells stained with AAA27030 (red line) at 1:100. The cells were incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-All HA-Tag Monoclonal Antibody
HA-Tag Monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IF: 1:50-1:200
IP: 1ug-5ug
FC: 1:50-1:200
FCM (Flow Cytometry)
(Overlay histogram showing 293F transfected cells stained with AAA27030 (red line) at 1:100. The cells were incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing 293F transfected cells stained with AAA27030 (red line) at 1:100. The cells were incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
IP (Immunoprecipitation)
(Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27030 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (5ug), Company B (5ug),Company C (5ug),Company D (5ug),Company E (5ug), (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27030 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (5ug), Company B (5ug),Company C (5ug),Company D (5ug),Company E (5ug), (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27030 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA27030 (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HA-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27030 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA27030 (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, AAA27030 at 1:100, counter- stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, AAA27030 at 1:100, counter- stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F transfected cells with AAA27030 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F transfected cells with AAA27030 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells with AAA27030 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells with AAA27030 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
WB (Western Blot)
(Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate All lanes: HA-Tag antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution)
WB (Western Blot)
(Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate All lanes: HA-Tag antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution)
WB (Western Blot)
(Western BlotPositive WB detected in: HA-tagged fusion proteinAll lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: HA-tagged fusion proteinAll lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ngAll lanes: HA-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ngAll lanes: HA-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 3 different overexpression lysates with HA taggedAll lanes: HA-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35, 35, 48 kDaObserved band size: 35, 35, 48 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 3 different overexpression lysates with HA taggedAll lanes: HA-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35, 35, 48 kDaObserved band size: 35, 35, 48 kDa)
NCBI and Uniprot Product Information
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Product Notes
The HA-Tag (Catalog #AAA27030) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HA-Tag Monoclonal Antibody reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's HA-Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunoprecipitation (IP). WB: 1:5000-1:160000 IF: 1:50-1:200 IP: 1ug-5ug FC: 1:50-1:200. Researchers should empirically determine the suitability of the HA-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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