HdmX/MDM4 recombinant antibody

Rabbit anti-HdmX/MDM4 Recombinant Monoclonal Antibody [BL-3-2F2]

Cat. Num. AAA213529

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: HdmX/MDM4, Recombinant Antibody; Rabbit anti-HdmX/MDM4 Recombinant Monoclonal Antibody [BL-3-2F2]; MDMX; protein Mdm4; double minute 4, human homolog of; p53-binding protein Mdm4; protein Mdmx; p53-binding protein; MRP1; MDM4 protein variant Y; MDM4 protein variant G; Mdm4 p53 binding protein homolog; HDMX; Double minute 4 protein; mdm2-like p53-binding protein; MDM4-related protein 1; Protein Mdm4; MDM4, p53 regulator; HdmX/MDM4 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: BL-3-2F2
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for HdmX/MDM4 recombinant antibody: WB (Simple Western Blot), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 125 and 175
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, MCF-7, RKO, U2OS, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (AAA213529 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

Simple Western

(Detection of human HdmX/MDM4 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (AAA213529) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation)

(Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (AAA213529 lot 4) used for IP at 20 ul/mg lysate. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (AAA213529 lot 3) and a second antibody against a different epitope of HdmX/MDM4 (BL15229). For blotting immunoprecipitated HdmX/MDM4, AAA213529 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

FCM/FACS (Flow Cytometry)

(Detection of human HdmX/MDM4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (AAA213529) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for HdmX/MDM4 recombinant antibody

HdmX/MDM4 is a RING-finger domain containing protein that interacts with p53. HdmX/MDM4 may influence p53 stability through an interaction with the structurally similar MDM2, an ubiquitin-ligase that targets p53 for proteasomal degradation. HdmX/MDM4 may influence p53 by directly attenuating p53 transcriptional activity.

Product Categories/Family for HdmX/MDM4 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 88702791
• NCBI GeneID: 4194
• NCBI Accession #: NP_002384.2
• NCBI GenBank Nucleotide #: NM_002393.4
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 49,541 Da
• NCBI Official Full Name: protein Mdm4 isoform 1
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4; protein Mdmx; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; double minute 4, human homolog of; p53-binding protein
• UniProt Protein Name: Protein Mdm4
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX
• UniProt Entry Name: MDM4_HUMAN