WB (Western Blot)
(Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody (AAA210863 lot 5) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human HdmX/MDM4 Polyclonal Antibody | anti-HdmX/MDM4 antibody
Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified
Gene Names
MDM4; HDMX; MDMX; MRP1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HdmX/MDM4, Antibody; Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified; MDMX; protein Mdm4; double minute 4, human homolog of; p53-binding protein Mdm4; protein Mdmx; p53-binding protein; MRP1; MDM4 protein variant Y; MDM4 protein variant G; Mdm4 p53 binding protein homolog; HDMX; Double minute 4 protein; mdm2-like p53-binding protein; MDM4-related protein 1; Protein Mdm4; MDM4, p53 regulator; anti-HdmX/MDM4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HdmX/MDM4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody (AAA210863 lot 5) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody (AAA210863 lot 5) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody (AAA210863 lot 5) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (AAA210863 lot 4) and a second antibody against a different epitope of HdmX/MDM4 . For blotting immunoprecipitated HdmX/MDM4, AAA210863 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody (AAA210863 lot 5) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (AAA210863 lot 4) and a second antibody against a different epitope of HdmX/MDM4 . For blotting immunoprecipitated HdmX/MDM4, AAA210863 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-HdmX/MDM4 antibody
HdmX/MDM4 is a RING-finger domain containing protein that interacts with p53. HdmX/MDM4 may influence p53 stability through an interaction with the structurally similar MDM2, an ubiquitin-ligase that targets p53 for proteasomal degradation. HdmX/MDM4 may influence p53 by directly attenuating p53 transcriptional activity.
Product Categories/Family for anti-HdmX/MDM4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,541 Da
NCBI Official Full Name
protein Mdm4 isoform 1
NCBI Official Synonym Full Names
MDM4, p53 regulator
NCBI Official Symbol
MDM4
NCBI Official Synonym Symbols
HDMX; MDMX; MRP1
NCBI Protein Information
protein Mdm4; protein Mdmx; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; double minute 4, human homolog of; p53-binding protein
UniProt Protein Name
Protein Mdm4
UniProt Gene Name
MDM4
UniProt Synonym Gene Names
MDMX
UniProt Entry Name
MDM4_HUMAN
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Product Notes
The HdmX/MDM4 mdm4 (Catalog #AAA210863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HdmX/MDM4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HdmX/MDM4 mdm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HdmX/MDM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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