Mouse anti-Human Heat Shock 27kDa Protein 1 Monoclonal Antibody | anti-HSPB1 antibody

Heat Shock 27kDa Protein 1 Recombinant Mouse Monoclonal Antibody [Clone rHSPB1/6489]

Cat. Num. AAA215707

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Heat Shock 27kDa Protein 1, Antibody; Heat Shock 27kDa Protein 1 Recombinant Mouse Monoclonal Antibody [Clone rHSPB1/6489]; Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27); anti-HSPB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a, kappa
• Clone Number: rHSPB1/6489
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-HSPB1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant human full-length HSP27 protein
• Cellular Localization: Predominantly cytoplasmic with some nuclear. It is cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub
• Positive Control: MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR
• Hu-chromosome Location: 7q11.23
• Unigene: 520973
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified HSP27 Recombinant Mouse Monoclonal Antibody (rHSPB1/6489). Confirmation of Purity and Integrity of Antibody.)

Related Product Information for anti-HSPB1 antibody

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 4504517
• NCBI GeneID: 3315
• NCBI Accession #: NP_001531.1
• NCBI GenBank Nucleotide #: NM_001540.3
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock 27kDa protein 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
• NCBI Protein Information: heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN