Rabbit [pSer82]HSP27 Polyclonal Antibody | anti-HspB1; Heat shock protein 27 antibody

HspB1, Heat shock protein 27

Cat. Num. AAA77006

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Sheep
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Synonyms: [pSer82]HSP27, Antibody; HspB1, Heat shock protein 27; HSP27; anti-HspB1; Heat shock protein 27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Sheep
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, guinea pig and sheep HSP27. Detects a band of ~27kDa by Western blot.
• Form/Format: Liquid. Whole rabbit serum.
• Sequence Length: 205

• Applicable Applications for anti-HspB1; Heat shock protein 27 antibody: WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence)
• Immunogen: Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser82.
• Preparation and Storage: Long term: Serum should be stored at -20 degree C.; Short term storage: 4 degree C, supplement with 0.1% sodium azide.; Avoid freeze/thaw cycles.; Shipping: Ships on blue ice.

IF (Immunofluorescence)

(Immunofluorescent analysis (confocal) staining of HeLa cells using Hsp27 (pSer82) pAb (green); nuclei are stained in blue pseudocolor using DRAQ5)

WB (Western Blot)

(Western blot analysis of phosphorylated HSP27 and nonphosphorylated HSP27 proteins probed with HSP27 (pSer82) pAb)

Related Product Information for anti-HspB1; Heat shock protein 27 antibody

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Product Categories/Family for anti-HspB1; Heat shock protein 27 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 19855073
• NCBI GeneID: 3315
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: Heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN