IHC (Immunohiostchemistry)
(IHC: Human breastcarcinoma tissue stainedwith Rat anti HSF1 antibody,at 1:100 for10 min @ RT. Staining offormalin-fixed tissuerequires boiling tissuesections in 10 mM CitrateBuffer, pH 6.0 for 10 minfollowed by cooling at RT for20 min.)
Rat Heat Shock Factor 1 Monoclonal Antibody | anti-HSF1 antibody
Rat Anti Heat Shock Factor 1 Monoclonal Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA
Purity
Purified by Affinity Chromatography according to the isotyping.
Synonyms
Heat Shock Factor 1, Antibody; Rat Anti Heat Shock Factor 1 Monoclonal Antibody; Rat anti HSF1 Monoclonal Antibody; Heat shock factor protein 1; Heat Shock Factor 1 (HSF1); Heat shock transcription factor (HSTF1); Rat anti-HSF1; anti-HSF1 antibody
Host
Rat
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1k
Specificity
This antibody recognizes GST-fusion proteins.
Purity/Purification
Purified by Affinity Chromatography according to the isotyping.
Form/Format
Supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Sequence Length
529
Applicable Applications for anti-HSF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA
Antigen Preparation
A recombinant mouse HSF1 (1-503aa) protein.
Positive Control
LS174T cells. Breast carcinoma.
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohiostchemistry)
(IHC: Human breastcarcinoma tissue stainedwith Rat anti HSF1 antibody,at 1:100 for10 min @ RT. Staining offormalin-fixed tissuerequires boiling tissuesections in 10 mM CitrateBuffer, pH 6.0 for 10 minfollowed by cooling at RT for20 min.)
IHC (Immunohiostchemistry)
(IHC: Human breastcarcinoma tissue stainedwith Rat anti HSF1 antibody,at 1:100 for10 min @ RT. Staining offormalin-fixed tissuerequires boiling tissuesections in 10 mM CitrateBuffer, pH 6.0 for 10 minfollowed by cooling at RT for20 min.)
WB (Western Blot)
(Western Blot: The cell lysatewas derived from MCF7 wasresolved onto 7.5% SDSPAGE, transferred onto NCmembrane, andimmunoprobed by Rat antiHSF1 at1:500 dilution. A majorimmunoreactive band isobserved around 70kDa.)
WB (Western Blot)
(Western Blot: The cell lysatewas derived from MCF7 wasresolved onto 7.5% SDSPAGE, transferred onto NCmembrane, andimmunoprobed by Rat antiHSF1 at1:500 dilution. A majorimmunoreactive band isobserved around 70kDa.)
Related Product Information for anti-HSF1 antibody
Heat-shock factor 1 (HSF1) is the master transcriptional regulator to respond to the cellular stress signals, such as heat, heavy metals, and oxidative reagents and a wide variety of other stressors. It binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. The increased HSF1 which is associated with reduced breast cancer survival may imply that HSF1 should be evaluated prospectively as an independent prognostic indicator in ER-positive breast cancer. HSF1 may ultimately be a useful therapeutic target in cancer.
References
Nunes,S.L. and Calderwood,S.K. Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells. Biochem. Biophys. Res. Commun. 213 (1), 1-6 (1995).
NCBI and Uniprot Product Information
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Product Notes
The HSF1 (Catalog #AAA71351) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Heat Shock Factor 1 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Factor 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HSF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Factor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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