IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
Mouse anti-Human HEG1 Monoclonal Antibody | anti-HEG1 antibody
HEG1 (SKM9-2)
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
HEG1, Antibody; HEG1 (SKM9-2); heg1; heg 1; heg-1; anti-heg1; anti-heg 1; anti-heg-1; Sialylated protein HEG homolog 1; Heart development protein with EGF like domains 1; HEG; HEG homolog 1 zebrafish; HEG1; Protein HEG homolog 1; anti-HEG1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
SKM9-2
Form/Format
HEG1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH7.5, containing BSA and sodium azide as a preservative
Applicable Applications for anti-HEG1 antibody
IHC (Immunohistochemistry)
Immunogen
893-SKSPSLVSLPT-903 of the human HEG1 protein.
Control
Breast, Fallopian Tube, Colon, Prostate, Testis, Transitional Cell Carcinoma, Papillary Thyroid Carcinoma, Colon Adenocarcinoma
Localization
Cytoplasmic
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
3. Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
3. Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
Preparation and Storage
Store at 2-8°C (Control Slides: Store at 20-25°C)
IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
IHC (Immunohiostchemistry)
(HEG1 Antibody Immunohistochemistry on an FFPE Mesothelioma Tissue)
IHC (Immunohiostchemistry)
(HEG1 Antibody Immunohistochemistry on an FFPE Mesothelioma Tissue)
Dilution Info
Dilution Info
Related Product Information for anti-HEG1 antibody
Intended Use: This antibody is intended for use in Immunohistochemical applications on formallin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations.
Sialylated protein HEG homolog 1 or Heart development protein with EGF like domains 1 (HEG1) is a novel calcium binding receptor protein involved in the Cerebral Cavernous Malformations (CCM) pathway and plays a regulatory role in heart and vessel formation by stabilizing endothelial cell junctions. HEG1 is a heavily glycosylated protein that participates in endothelial cell associations that develop the vascular system, and therefore plays a role in angiogenesis and the microenvironment of cellular adhesion.
The exact role of HEG1 in hepatocellular carcinoma is unclear, however, studies have shown HEG1 antibody expression indicates poor prognosis and plays important roles in hepatocellular carcinoma invasion, metastasis, and epithelial mesenchymal transition by activating Wnt/beta-catenin signaling. HEG1 mucin-like membrane protein has also been found to be a sensitive and specific biomarker for malignant mesothelioma in pleural (lung) and peritoneal (abdominal) membranes, and several other rare locations, where it supports the survival and proliferation of mesothelioma cells. HEG1 is potentially a useful prognostic biomarker and therapeutic target for malignant mesothelioma and hepatocellular, thyroid, and ovarian carcinomas.
HEG1 antibody has been found to be a highly specific and sensitive marker of epithelioid Malignant Mesothelioma and offers sensitivity comparable to conventional markers for Epithelioid Mesotheliomas, but provides considerably better specificity, such that the identification of epithelioid mesothelioma versus NSCLC and potentially could be confirmed with a combination of HEG1 antibody and a suitable broad spectrum carcinoma marker such as Claudin-4.
Sialylated protein HEG homolog 1 or Heart development protein with EGF like domains 1 (HEG1) is a novel calcium binding receptor protein involved in the Cerebral Cavernous Malformations (CCM) pathway and plays a regulatory role in heart and vessel formation by stabilizing endothelial cell junctions. HEG1 is a heavily glycosylated protein that participates in endothelial cell associations that develop the vascular system, and therefore plays a role in angiogenesis and the microenvironment of cellular adhesion.
The exact role of HEG1 in hepatocellular carcinoma is unclear, however, studies have shown HEG1 antibody expression indicates poor prognosis and plays important roles in hepatocellular carcinoma invasion, metastasis, and epithelial mesenchymal transition by activating Wnt/beta-catenin signaling. HEG1 mucin-like membrane protein has also been found to be a sensitive and specific biomarker for malignant mesothelioma in pleural (lung) and peritoneal (abdominal) membranes, and several other rare locations, where it supports the survival and proliferation of mesothelioma cells. HEG1 is potentially a useful prognostic biomarker and therapeutic target for malignant mesothelioma and hepatocellular, thyroid, and ovarian carcinomas.
HEG1 antibody has been found to be a highly specific and sensitive marker of epithelioid Malignant Mesothelioma and offers sensitivity comparable to conventional markers for Epithelioid Mesotheliomas, but provides considerably better specificity, such that the identification of epithelioid mesothelioma versus NSCLC and potentially could be confirmed with a combination of HEG1 antibody and a suitable broad spectrum carcinoma marker such as Claudin-4.
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Product Notes
The HEG1 (Catalog #AAA59372) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HEG1 (SKM9-2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEG1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HEG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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