Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse HER-4/ERBB4 Monoclonal Antibody | anti-ERBB4 antibody
HER-4/ERBB4
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
HER-4/ERBB4, Antibody; HER-4/ERBB4; Avian erythroblastic leukemia viral oncogene homolog 4; ERBB4; HER4; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4; anti-ERBB4 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
ERBB4/2582
Specificity
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1,298
Applicable Applications for anti-ERBB4 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)
Positive Control
Breast, Brain, Heart or Kidney
Cellular Localization
Cytoplasmic, Cell Surface and Nuclear
Chromosome Location
2q34
Preparation and Storage
Antibody without azide - store at -20 to -80°C.
Antibody is stable for 24 months.
Non-hazardous. No MSDS required.
Antibody is stable for 24 months.
Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).)
References
Carpenter, G. (2003) Exp Cell Res 284, 66-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180kDa (precursor); 80/120kDa (cleaved)
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-2
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 4
NCBI Official Symbol
ERBB4
NCBI Official Synonym Symbols
HER4; ALS19; p180erbB4
NCBI Protein Information
receptor tyrosine-protein kinase erbB-4
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-4
UniProt Gene Name
ERBB4
UniProt Synonym Gene Names
HER4; 4ICD; E4ICD
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Product Notes
The ERBB4 erbb4 (Catalog #AAA214736) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HER-4/ERBB4 reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's HER-4/ERBB4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ERBB4 erbb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HER-4/ERBB4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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