FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).)
Mouse anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody | anti-HDAC2 antibody
Histone Deacetylase 2/HDAC2
Gene Names
HDAC2; HD2; RPD3; YAF1; KDAC2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
Protein-A Affinity Chromatography
Synonyms
Histone Deacetylase 2/HDAC2, Antibody; Histone Deacetylase 2/HDAC2; Histone deacetylase 2; HD2; RPD3; YAF1; anti-HDAC2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT3B1
Purity/Purification
Protein-A Affinity Chromatography
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
488
Applicable Applications for anti-HDAC2 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human HDAC2 (1-488aa) purified from E Coli.
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).)
WB (Western Blot)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human HDAC2)
WB (Western Blot)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human HDAC2)
Related Product Information for anti-HDAC2 antibody
HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Product Categories/Family for anti-HDAC2 antibody
References
Betz R. et al. (1998) Genomics. 52:245-6. Taunton J. et al. (1996) Science 272:408-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histone deacetylase 2
NCBI Official Synonym Full Names
histone deacetylase 2
NCBI Official Symbol
HDAC2
NCBI Official Synonym Symbols
HD2; RPD3; YAF1; KDAC2
NCBI Protein Information
histone deacetylase 2
UniProt Protein Name
Histone deacetylase 2
UniProt Gene Name
HDAC2
UniProt Synonym Gene Names
HD2
UniProt Entry Name
HDAC2_HUMAN
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Product Notes
The HDAC2 hdac2 (Catalog #AAA48277) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone Deacetylase 2/HDAC2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone Deacetylase 2/HDAC2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HDAC2 hdac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 2/HDAC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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