ICC (Immunocytochemistry)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
Mouse anti-Human, Mouse Histone H2A.X Monoclonal Antibody | anti-H2AFX antibody
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Synonyms
Histone H2A.X, Antibody; Phospho-Histone H2A.X (Ser139) Antibody; Purified Mouse Monoclonal Antibody (Mab); H2A histone family; member X; H2A.X; H2a/x; H2AFX; H2AX; H2AX histone; H2AX_HUMAN; Histone H2A.X; Histone H2AX; anti-H2AFX antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Sequence Length
143
Applicable Applications for anti-H2AFX antibody
ICC (Immunocytochemistry), WB (Western Blot)
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
WB (Western Blot)
(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
WB (Western Blot)
(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
Related Product Information for anti-H2AFX antibody
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
References
Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989). Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998). Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999). Paull T.T.,et al.Curr. Biol. 10:886-895(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15145
NCBI Official Full Name
histone H2AX
NCBI Official Synonym Full Names
H2A histone family member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2AX
UniProt Protein Name
Histone H2AX
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2AFX h2afx (Catalog #AAA290456) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Histone H2A.X (Ser139) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.X can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the H2AFX h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.X, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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