WB (Western Blot)
(Histone H2AX pAb tested by Western blot. 30 ug of HeLa cell nuclear extract was probed with Histone H2AX pAb at a dilution of 1:2,000.)
Rabbit anti-Human Histone H2A.X Polyclonal Antibody | anti-H2A.X antibody
Histone H2A.X antibody (pAb)(H2AX)
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Histone H2A.X, Antibody; Histone H2A.X antibody (pAb)(H2AX); anti-H2A.X antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H2A.X antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
Immunogen
This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Histone H2AX pAb tested by Western blot. 30 ug of HeLa cell nuclear extract was probed with Histone H2AX pAb at a dilution of 1:2,000.)
WB (Western Blot)
(Histone H2AX pAb tested by Western blot. 30 ug of HeLa cell nuclear extract was probed with Histone H2AX pAb at a dilution of 1:2,000.)
WB (Western Blot)
(Histone H2AX pAb tested by Western blot. Western blot probed with Histone H2AX pAb at a dilution of 1:2,000. Lane 1: Recombinant human histone H2AX (100 ng). Lane 2: Mouse embryonic fibroblast (MEF) cell wild type nuclear lysate. Lane 3: MEF cell nuclear extract derived from H2AX -/- nulls.)
WB (Western Blot)
(Histone H2AX pAb tested by Western blot. Western blot probed with Histone H2AX pAb at a dilution of 1:2,000. Lane 1: Recombinant human histone H2AX (100 ng). Lane 2: Mouse embryonic fibroblast (MEF) cell wild type nuclear lysate. Lane 3: MEF cell nuclear extract derived from H2AX -/- nulls.)
IF (Immunofluorescence)
(Histone H2AX antibody tested by immunofluorescence. Detection of Histone H2AX by immunofluorescence. HeLa cells were stained with Histone H2AX antibody at a dilution of 1:1,000. Top panel: Histone H2AX antibody staining. Middle panel: DAPI. Bottom panel: merge.)
IF (Immunofluorescence)
(Histone H2AX antibody tested by immunofluorescence. Detection of Histone H2AX by immunofluorescence. HeLa cells were stained with Histone H2AX antibody at a dilution of 1:1,000. Top panel: Histone H2AX antibody staining. Middle panel: DAPI. Bottom panel: merge.)
Related Product Information for anti-H2A.X antibody
Short Description: Our Histone H2A.X antibody (pAb)(H2AX) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples.
Background: Histone H2AX (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.
Background: Histone H2AX (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.
Product Categories/Family for anti-H2A.X antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2AX
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2AX; H2AX histone; histone H2A.x
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2A.X h2afx (Catalog #AAA59916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A.X antibody (pAb)(H2AX) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.X can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H2A.X h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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