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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).)
Rabbit Histone H2A.X (Ser139) Monoclonal Antibody | anti-H2A.X antibody
Histone H2A.X, phosphorylated (Ser139)
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Monkey, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
Histone H2A.X (Ser139); Monoclonal Antibody; Histone H2A.X; phosphorylated (Ser139); phosphorylated; anti-H2A.X antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
20000
Specificity
Recognizes endogenous levels of human H2A.X only when phosphorylated at serine 139. Species Crossreactivity: mouse, rat and monkey
Purity/Purification
Purified
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-H2A.X antibody
Flow Cytometry (FC/FACS), Immunochemistry (IC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
immunofluorescence (IF): 1:400
Flow Cytometry: 1:400
Western Blot: 1:1000
Immunohistochemistry: 1:480. Use citrate as unmasking buffer.
Flow Cytometry: 1:400
Western Blot: 1:1000
Immunohistochemistry: 1:480. Use citrate as unmasking buffer.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Modification
Phosphorylated
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma untreated (left) or lambda-phosphatase-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma untreated (left) or lambda-phosphatase-treated (right), using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, AAA26905, showing nuclear localization.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, AAA26905, showing nuclear localization.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AAA26905.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AAA26905.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using AAA26905 in the presence of control peptide (left) or Phospho-Histone H2A.X (Ser139) Blocking Peptide (right).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using AAA26905 in the presence of control peptide (left) or Phospho-Histone H2A.X (Ser139) Blocking Peptide (right).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded HT-29 cells untreated (left) or UV-treated (right), using AAA26905.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded HT-29 cells untreated (left) or UV-treated (right), using AAA26905.)
WB (Western Blot)
(Western Blot analysis of extracts from untreated or UV-treated 293 cells, using AAA26905 (upper) or Histone H2A.X Antibody (lower).)
WB (Western Blot)
(Western Blot analysis of extracts from untreated or UV-treated 293 cells, using AAA26905 (upper) or Histone H2A.X Antibody (lower).)
Related Product Information for anti-H2A.X antibody
Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts. H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks. DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK. Within minutes following DNA damage, H2A.X is phosphorylated at Ser139 at sites of DNA damage. This very early event in the DNA-damage response is required for recruitment of a multitude of DNA-damage response proteins, including MDC1, NBS1, RAD50, MRE11, 53BP1, and BRCA1.
Product Categories/Family for anti-H2A.X antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2A.x
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2A.x; H2AX histone
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2A.X h2afx (Catalog #AAA26905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A.X, phosphorylated (Ser139) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.X (Ser139) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunochemistry (IC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). immunofluorescence (IF): 1:400 Flow Cytometry: 1:400 Western Blot: 1:1000 Immunohistochemistry: 1:480. Use citrate as unmasking buffer. Researchers should empirically determine the suitability of the H2A.X h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.X (Ser139), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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