Application Data
(Histone H3 di/trimethyl Lys27 antibody (mAb) specificity data. HeLa acid extract (10 ug per lane) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500) with or without pre-incubation of antibody with 1 uM peptide containing the sequence surrounding either Lys9 or Lys27 of Histone H3. Lane 1: no peptide Lane 2: unmodified Lys27 Lane 3: monomethyl-Lys27 Lane 4: dimethyl Lys27 Lane 5: trimethyl-Lys27 Lane 6: unmodified Lys9 Lane 7: monomethyl-Lys9 Lane 8: dimethyl-Lys9 Lane 9: trimethyl-Lys9.)
Mouse Histone H3K27me2me3 Monoclonal Antibody | anti-H3K27me2me3 antibody
Histone H3K27me2me3 antibody (mAb)(Clone 7B11)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H3K27me2me3, Antibody; Histone H3K27me2me3 antibody (mAb)(Clone 7B11); anti-H3K27me2me3 antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG
Clone Number
7B11
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 10mM sodium phosphate (pH7.5), 150mM NaCl, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an ascites version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K27me2me3 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
Conjugation
Methylated
Immunogen
This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(Histone H3 di/trimethyl Lys27 antibody (mAb) specificity data. HeLa acid extract (10 ug per lane) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500) with or without pre-incubation of antibody with 1 uM peptide containing the sequence surrounding either Lys9 or Lys27 of Histone H3. Lane 1: no peptide Lane 2: unmodified Lys27 Lane 3: monomethyl-Lys27 Lane 4: dimethyl Lys27 Lane 5: trimethyl-Lys27 Lane 6: unmodified Lys9 Lane 7: monomethyl-Lys9 Lane 8: dimethyl-Lys9 Lane 9: trimethyl-Lys9.)
Application Data
(Histone H3 di/trimethyl Lys27 antibody (mAb) specificity data. HeLa acid extract (10 ug per lane) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500) with or without pre-incubation of antibody with 1 uM peptide containing the sequence surrounding either Lys9 or Lys27 of Histone H3. Lane 1: no peptide Lane 2: unmodified Lys27 Lane 3: monomethyl-Lys27 Lane 4: dimethyl Lys27 Lane 5: trimethyl-Lys27 Lane 6: unmodified Lys9 Lane 7: monomethyl-Lys9 Lane 8: dimethyl-Lys9 Lane 9: trimethyl-Lys9.)
WB (Western Blot)
(Histone H3 di/trimethyl Lys27 antibody (mAb) tested by Western blot. HeLa acid extract (10 ug) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500).)
WB (Western Blot)
(Histone H3 di/trimethyl Lys27 antibody (mAb) tested by Western blot. HeLa acid extract (10 ug) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500).)
IF (Immunofluorescence)
(Histone H3 di/trimethyl Lys27 antibody (mAb) tested by immunofluorescence. Top: HeLa cells stained with Histone H3 di/trimethyl Lys27 antibody (mAb) at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.)
IF (Immunofluorescence)
(Histone H3 di/trimethyl Lys27 antibody (mAb) tested by immunofluorescence. Top: HeLa cells stained with Histone H3 di/trimethyl Lys27 antibody (mAb) at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.)
Related Product Information for anti-H3K27me2me3 antibody
Short Description: Our Histone H3K27me2me3 antibody (mAb)(Clone 7B11) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.
Product Categories/Family for anti-H3K27me2me3 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K27me2me3 (Catalog #AAA59876) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K27me2me3 antibody (mAb)(Clone 7B11) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K27me2me3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H3K27me2me3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K27me2me3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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