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Rabbit Histone H4 Monoclonal Antibody | anti-HIST2H4B antibody
Histone H4 (Acetyl K16) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Histone H4, Antibody; Histone H4 (Acetyl K16) Antibody; Histone gene cluster 1; H4A antibody; Histone gene cluster 2; H4 antibody; dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16.1 antibody; dJ221C16.9 antibody; FO108 antibody; H4 histone family; member A antibody; member B antibody; member C antibody; member D antibody; member E antibody; member G antibody; member H antibody; member I antibody; member J antibody; member K antibody; member M antibody; member N antibody; H4 histone; family 2 antibody; H4/A antibody; H4/B antibody; H4/C antibody; H4/D antibody; H4/E antibody; H4/G antibody; H4/H antibody; H4/I antibody; H4/J antibody; H4/K antibody; H4/M antibody; H4/N antibody; H4/O antibody; H4/p antibody; H4_HUMAN antibody; H4F2 antibody; H4F2iii antibody; H4F2iv antibody; H4FA antibody; H4FB antibody; H4FC antibody; H4FD antibody; H4FE antibody; H4FG antibody; H4FH antibody; H4FI antibody; H4FJ antibody; H4FK antibody; HIST1 cluster; H4B antibody; H4D antibody; HIST2H4 antibody; Hist4 cluster; Hist4h4 antibody; histone 1; H4a antibody; H4c antibody; H4d antibody; H4f antibody; H4h antibody; H4i antibody; H4j antibody; H4k antibody; H4l antibody; histone 2; H4b antibody; Histone 4 family; histone 4; histone cluster 1; H4e antibody; histone cluster 2; histone cluster 4; Histone family; member L antibody; H4E antibody; H4K antibody; Histone gene cluster 4; H4 histone antibody; Histone H4 antibody; histone IV; anti-HIST2H4B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB21-44
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HIST2H4B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H4 (acetyl K16) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Histone H4 (acetyl K16) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Histone H4 (acetyl K16) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.)
Related Product Information for anti-HIST2H4B antibody
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Product Categories/Family for anti-HIST2H4B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 2, H4b
NCBI Official Symbol
HIST2H4B
NCBI Official Synonym Symbols
H4/o
NCBI Protein Information
histone H4; histone 2, H4b
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
