IF (Immunofluorescence)
(Immunofluorescent staining of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal (HLA-DQA1/2866R) followed by goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
Mouse HLA-DQ Monoclonal Antibody | anti-HLA-DQ antibody
HLA-DQ (MHC II)
Gene Names
HLA-DQA1; DQA1; DQ-A1; CELIAC1; HLA-DQA
Reactivity
Human, Pig. Others not known.
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
HLA-DQ, Antibody; HLA-DQ (MHC II); CELIAC1; DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE); HLA class II histocompatibility antigen DQ(1) alpha chain; HLA class II histocompatibility antigen DQ(3) alpha chain; HLA DQA1; HLA DQB1; IDDM1; Leucocyte antigen DQA1; Major histocompatibility complex class II DQ alpha 1; Major histocompatibility complex class II DQ beta 1; anti-HLA-DQ antibody
Host
Mouse
Reactivity
Human, Pig. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
HLA-DQA1/2866R
Specificity
Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. with in the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
254
Applicable Applications for anti-HLA-DQ antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant full-length human HLA-DQ protein
Positive Control
Raji Cells. Tonsil or Lymph Node
Cellular Localization
Cell Surface
Chromosome Location
6p21.3
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IF (Immunofluorescence)
(Immunofluorescent staining of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal (HLA-DQA1/2866R) followed by goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
IF (Immunofluorescence)
(Immunofluorescent staining of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal (HLA-DQA1/2866R) followed by goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal Antibody (HLA-DQA1/2866R) followed by goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal Antibody (HLA-DQA1/2866R) followed by goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).)
SDS-PAGE
(SDS-PAGE Analysis Purified HLA-DQ Rabbit Recombinant Monoclonal Ab (HLA-DQA1/2866R). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified HLA-DQ Rabbit Recombinant Monoclonal Ab (HLA-DQA1/2866R). Confirmation of Purity and Integrity of Antibody.)
Related Product Information for anti-HLA-DQ antibody
Recombinant Rabbit Monoclonal Antibody
References
Spits H, et al. Hybridoma, 1983, 2(4):423-37.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60kDa
NCBI Official Full Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DQ alpha 1
NCBI Official Symbol
HLA-DQA1
NCBI Official Synonym Symbols
DQA1; DQ-A1; CELIAC1; HLA-DQA
NCBI Protein Information
HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Protein Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Gene Name
HLA-DQA1
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Product Notes
The HLA-DQ hla-dqa1 (Catalog #AAA214758) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HLA-DQ (MHC II) reacts with Human, Pig. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-DQ can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the HLA-DQ hla-dqa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-DQ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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