FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells. HLA-DQ Mouse Monoclonal Antibody (SPM422) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Mouse anti-Human, Pig HLA-DQ Monoclonal Antibody | anti-HLA-DQ antibody
HLA-DQ (MHC II) Mouse Monoclonal Antibody
Gene Names
HLA-DQA1; CD; GSE; DQ-A1; CELIAC1; HLA-DQA
Reactivity
Human, Pig
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
HLA-DQ, Antibody; HLA-DQ (MHC II) Mouse Monoclonal Antibody; CELIAC1; DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE); HLA Class II DQ; HLA class II histocompatibility antigen DQ; HLA class II histocompatibility antigen DQ(1) alpha chain; HLA class II histocompatibility antigen DQ(3) alpha chain; HLA DQA1; HLA DQB; HLA DQB1; IDDM1; Leucocyte antigen DQA1; Major histocompatibility complex class II DQ alpha 1; Major histocompatibility complex class II DQ beta 1; MHC class II antigen HLA DQ beta 1; MHC class II HLA DQ alpha 1; MHC class II HLA DQ beta glycoprotein; MHC DQ beta; MHC HLA DQ alpha; anti-HLA-DQ antibody
Host
Mouse
Reactivity
Human, Pig
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM422
Specificity
Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
255
Applicable Applications for anti-HLA-DQ antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cell Surface
Immunogen
T4-positive CTL clone HG-38
Hu-Chromosome Location
6p21.3
Positive Control
Raji cells. Tonsil or lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells. HLA-DQ Mouse Monoclonal Antibody (SPM422) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells. HLA-DQ Mouse Monoclonal Antibody (SPM422) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
References
Spits H; Keizer G; Borst J; Terhorst C; Hekman A; de Vries JE. Characterization of monoclonal antibodies against cell surface molecules associated with cytotoxic activity of natural and activated killer cells and cloned CTL lines. Hybridoma, 1983, 2(4):423-37. Spits H., Borst J., Giphan M., Coligan J., Terhorst C. and de Vries J., Eur. J. Immunol. 14, (1984). Bontrop R., Schreuder G., Mikalski E., van Miltenburg R. and Giphan M., Tissue Antigens, 27, (1986).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60kDa
NCBI Official Full Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DQ alpha 1
NCBI Official Symbol
HLA-DQA1
NCBI Official Synonym Symbols
CD; GSE; DQ-A1; CELIAC1; HLA-DQA
NCBI Protein Information
HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Protein Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Gene Name
HLA-DQA1
UniProt Entry Name
DQA1_HUMAN
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Product Notes
The HLA-DQ hla-dqa1 (Catalog #AAA62762) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HLA-DQ (MHC II) Mouse Monoclonal Antibody reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-DQ can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the HLA-DQ hla-dqa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-DQ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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