anti-Human, Swine HLA DQ Monoclonal Antibody

Mouse anti Human HLA DQ

Cat. Num. AAA14564

• Reactivity: Human, Swine
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: HLA DQ, Antibody; Mouse anti Human HLA DQ; anti-HLA DQ antibody

• Host: Mouse (Balb/c)
• Reactivity: Human, Swine
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: NKI(SPV)L3
• Specificity: NKI(SPV)L3 reacts with a monomorphic determinant on HLA-DQ (MHC class II).
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-HLA DQ antibody: Flow Cytometry (FC), Immunohistochemistry-Frozen (IHC-F), Immunoprecipitation (IP)
• Application Notes: NKI(SPV)L3 is used in research and Human cell separation for research or clinical appliCation. NKI(SPV)L3 is useful for immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration.
• Product Type: Primary Antibodies
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4 degree C. For prolonged storage prepare appropriate aliquots and store at or below -20 degree C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Related Product Information for anti-HLA DQ antibody

HLA-DQ (DQ) is a cell surface receptor type protein found on antigen presenting cells. DQ is an αβ heterodimer of the MHC Class II type. The α and β chains are encoded by HLA-DQA1 and HLA-DQB1, respectively. These two loci are adjacent to each other on chromosome 6p21.3. Both the α-chain and β-chain vary greatly. A person often produces two α-chain and two β-chain variants and thus 4 DQ isoforms. The DQ loci are in close genetic linkage to HLA-DR but less closely linked to HLA-DP, HLA-A, HLA-B and HLA-C. DQ functions on antigen presenting cells, and is an antigen presenting molecule. Different DQ isoforms can bind to and present different antigens to T-cells. In this process T-cells are stimulated to grow and can signal B-cells to produce antibodies. DQ functions in recognizing and presenting foreign antigens (proteins derived from potential pathogens). But DQ is also involved in recognizing common self-antigens and presenting those antigens to the immune system in order to develop tolerance from a very young age. When tolerance to self proteins is lost, DQ may become involved in autoimmune disease. Two autoimmune diseases in which HLA-DQ is involved are coeliac disease and diabetes mellitus type 1. DQ is one of several antigens involved in rejection of organ transplants. As a variable cell surface receptor on immune cells, these D antigens, originally HL-A4 antigens, are involved in graft versus host disease when lymphoid tissues are transplanted between people.

Product Categories/Family for anti-HLA DQ antibody

Haematology

References

Spits H., G. Keizer, J. Borst, C. Terhorst, A. Hekman and J.E. de Vries. Characterization of monoclonal antibodies anti cell surface molecules associated with cytotoxic activity of natural and activated killer cells and cloned CTL lines. Hybridoma 1983, 4: 423-437. Spits H., J. Borst, M. Giphart, J. Coligan, C. Terhorst and J.E. de Vries. HLA-DC antigens can serve as recognition elements for Human cytotoxic T lymphocytes. Eur. J. Immunol. 1984, 14: 299. A.A. te Velde, J.P.G. Klomp, B.A. Yard, J.E. de Vries, C.G. Figdor. Modulation of phenotypic and functional properties of Human peripheral blood monocytes by IL-4. J. of Immunol. 1988, 140: 1548-1554.