IHC (Immunohistochemisry)
(Immunohistochemical analysis of HLCS protein in paraffin embedded Carcinoma of Human prostate tissue using HLCS antibody)
Mouse HLCS Monoclonal Antibody | anti-HLCS antibody
HLCS antibody
Gene Names
Hlcs; D16Jhu34; 410I21.SP6
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
HLCS antibody was purified by affinity chromatography.
Synonyms
HLCS, Antibody; HLCS antibody; Monoclonal HLCS; Anti-HLCS; HCS; anti-HLCS antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1E4
Purity/Purification
HLCS antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
722
Applicable Applications for anti-HLCS antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Biological Significance
This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency.
Cross-Reactivity
Human
Immunogen
HLCS antibody was raised in Mouse using full length human recombinant protein of human HLCS produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of HLCS protein in paraffin embedded Carcinoma of Human prostate tissue using HLCS antibody)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of HLCS protein in paraffin embedded Carcinoma of Human prostate tissue using HLCS antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HLCS protein (Right) or empty vector (Left) detected with HLCS antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HLCS protein (Right) or empty vector (Left) detected with HLCS antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HLCS protein (red) or empty vector (blue) stained using HLCS antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HLCS protein (red) or empty vector (blue) stained using HLCS antibody)
Related Product Information for anti-HLCS antibody
Mouse monoclonal HLCS antibody
Product Categories/Family for anti-HLCS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
78,516 Da
NCBI Official Full Name
Hlcs
NCBI Official Synonym Full Names
holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
NCBI Official Symbol
Hlcs
NCBI Official Synonym Symbols
D16Jhu34; 410I21.SP6
NCBI Protein Information
biotin--protein ligase
UniProt Protein Name
Biotin--protein ligase
UniProt Gene Name
Hlcs
UniProt Synonym Gene Names
HCS
UniProt Entry Name
BPL1_MOUSE
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Product Notes
The HLCS hlcs (Catalog #AAA107377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HLCS can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HLCS hlcs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLCS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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