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ELISA
(Detection limit for recombinant GST tagged HMGB1 is approximately 3 ng/ml as a capture antibody.)
Mouse anti-Human HMG1 / HMGB1 Monoclonal Antibody | anti-HMG1 antibody
Mouse Monoclonal [clone 1B11] (IgG2a,k) to Human HMG1 / HMGB1
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
HMG1 / HMGB1; Monoclonal Antibody; Mouse Monoclonal [clone 1B11] (IgG2a; k) to Human HMG1 / HMGB1; Anti-HMG1 / HMGB1 Antibody (clone 1B11) IHC-plus; HMGB1; Amphoterin; High mobility group protein B1; High mobility group box 1; High mobility group protein 1; HMG1; HMG3; SBP-1; High-mobility group box 1; HMG-1; Human HMG1; anti-HMG1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1B11
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
215
Applicable Applications for anti-HMG1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. ELISA (Recombinant protein).
Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. ELISA (Recombinant protein).
Target Species
Human
Immunogen Description
HMGB1 (AAH03378, 1 a.a. ~ 216 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
HMG1 / HMGB1 antibody was raised against hMGB1 (AAH03378, 1 a.a. ~ 216 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Species
HMG1/ HMGB1 antibody was raised against Human.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
ELISA
(Detection limit for recombinant GST tagged HMGB1 is approximately 3 ng/ml as a capture antibody.)
ELISA
(Detection limit for recombinant GST tagged HMGB1 is approximately 3 ng/ml as a capture antibody.)
WB (Western Blot)
(Western blot of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody, clone 1B11.)
WB (Western Blot)
(Western blot of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody, clone 1B11.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell (antibody concentration 10 ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell (antibody concentration 10 ug/ml).)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HMGB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The HMG1 hmgb1 (Catalog #AAA12346) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 1B11] (IgG2a,k) to Human HMG1 / HMGB1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMG1 / HMGB1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. ELISA (Recombinant protein). Researchers should empirically determine the suitability of the HMG1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG1 / HMGB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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