WB (Western Blot)
(HNF-3a / FOXA1 antibody (mAb) tested by Western blot. Extract of HeLa cells over-expressing mouse HNF-3a / FOXA1 (50 ug per lane) probed with HNF-3b / FOXA1 antibody (mAb) (1:5,000 dilution).)
Mouse anti-Human, Mouse HNF-3a/FOXA1 Monoclonal Antibody | anti-HNF-3a/FOXA1 antibody
HNF-3a/FOXA1 antibody (mAb)(Clone 2F83)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
HNF-3a/FOXA1, Antibody; HNF-3a/FOXA1 antibody (mAb)(Clone 2F83); TCF3A; anti-HNF-3a/FOXA1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
2F83
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-HNF-3a/FOXA1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This HNF-3a/FOXA1 antibody was raised against a recombinant protein corresponding to amino acids 7-103 of rat HNF-3a /FOXA1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(HNF-3a / FOXA1 antibody (mAb) tested by Western blot. Extract of HeLa cells over-expressing mouse HNF-3a / FOXA1 (50 ug per lane) probed with HNF-3b / FOXA1 antibody (mAb) (1:5,000 dilution).)
WB (Western Blot)
(HNF-3a / FOXA1 antibody (mAb) tested by Western blot. Extract of HeLa cells over-expressing mouse HNF-3a / FOXA1 (50 ug per lane) probed with HNF-3b / FOXA1 antibody (mAb) (1:5,000 dilution).)
ChIP (Chromatin Immunoprecipitation)
(HNF-3a / FOXA1 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from LnCaP cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HNF-3a / FOXA1 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
ChIP (Chromatin Immunoprecipitation)
(HNF-3a / FOXA1 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from LnCaP cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HNF-3a / FOXA1 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Related Product Information for anti-HNF-3a/FOXA1 antibody
Short Description: Our HNF-3a/FOXA1 antibody (mAb)(Clone 2F83) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot; it has been shown to react with Human and Mouse samples.
Background: HNF-3a (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1a, HNF-1b, HNF-3a, HNF-3b, HNF-3g, HNF-4a, HNF-4g, HNF-6a and HNF-6b), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3a and HNF-3g have also been shown to be involved in gastrulation events such as body axis formation.
Background: HNF-3a (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1a, HNF-1b, HNF-3a, HNF-3b, HNF-3g, HNF-4a, HNF-4g, HNF-6a and HNF-6b), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3a and HNF-3g have also been shown to be involved in gastrulation events such as body axis formation.
Product Categories/Family for anti-HNF-3a/FOXA1 antibody
NCBI and Uniprot Product Information
Molecular Weight
50kDa
Similar Products
Product Notes
The HNF-3a/FOXA1 (Catalog #AAA59937) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HNF-3a/FOXA1 antibody (mAb)(Clone 2F83) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HNF-3a/FOXA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HNF-3a/FOXA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF-3a/FOXA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.