Mouse anti-Human hnRNP U Monoclonal Antibody | anti-HNRNPU antibody

hnRNP U Antibody

Cat. Num. AAA290476

• Gene Names: HNRNPU; SAFA; HNRPU; SAF-A; U21.1; hnRNP U; HNRNPU-AS1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: hnRNP U, Antibody; hnRNP U Antibody; Purified Mouse Monoclonal Antibody (Mab); Heterogeneous nuclear ribonucleoprotein U; hnRNP U protein; HNRNPU; hnRNPU protein; HNRPU; HNRPU_HUMAN; p120; p120 nuclear protein; SAF A; SAF-A; SAFA; Scaffold attachment factor A; U21.1.; anti-HNRNPU antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.09% (W/V) sodium azide, 50% glycerol
• Sequence Length: 806

• Applicable Applications for anti-HNRNPU antibody: WB (Western Blot)
• Function: DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability (PubMed: , PubMed: , PubMed: ). Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (By similarity). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner (PubMed: , PubMed: ). Blocks Pol II transcription elongation activity by inhibiting the C- terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation (PubMed: ). Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus (PubMed: ). Negatively regulates glucocorticoid-mediated transcriptional activation (PubMed: ). Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (By similarity). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression (PubMed: ). Participates in the circadian regulation of the core clock component BMAL1 transcription (By similarity). Plays a role in the regulation of telomere length (PubMed: ). Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis (PubMed: ). Plays a role in mRNA stability (PubMed: , PubMed: , PubMed: ). Component of the CRD-mediated complex that promotes MYC mRNA stabilization (PubMed: ). Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR) (PubMed: ). Plays a role in mitotic cell cycle regulation (PubMed: , PubMed: ). Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression (PubMed: ). Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis (PubMed: ). Contributes also to the targeting of AURKA to mitotic spindle MTs (PubMed: ). Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Binds to chromatin-associated RNAs (caRNAs) (PubMed: ). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Binds to the Xist RNA (PubMed: ). Binds the long non-coding H19 RNA (PubMed: ). Binds to SMN1/2 pre-mRNAs at G/U-rich regions (PubMed: ). Binds to small nuclear RNAs (snRNAs) (PubMed: ). Binds to the 3'-UTR of TNFA mRNA (PubMed: ). Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (By similarity). Required for embryonic development (By similarity). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (By similarity).
• Cellular Location: Nucleus. Nucleus matrix. Chromosome. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Midbody. Cytoplasm Cell surface. Cytoplasmic granule. Note=Localizes at inactive X chromosome (Xi) regions (PubMed:11003645, PubMed:14608463, PubMed:15563465) Localizes in the nucleus during interphase (PubMed:21242313). At metaphase, localizes with mitotic spindle microtubules (MTs) (PubMed:21242313). At anaphase, localizes in the mitotic spindle midzone (PubMed:21242313). Localizes in spindle MTs proximal to spindle poles in a TPX2- and AURKA-dependent manner (PubMed:21242313). The Ser- 59 phosphorylated form localizes to centrosomes during prophase and metaphase, to mitotic spindles in anaphase and to the midbody during cytokinesis (PubMed:25986610). Colocalizes with SMARCA4 in the nucleus (By similarity). Colocalizes with CBX5 in the nucleus (PubMed:19617346). Colocalizes with NR3C1 in nuclear speckles (PubMed:9353307). Localized in cytoplasmic ribonucleoprotein (RNP) granules containing untranslated mRNAs (PubMed:17289661) {ECO:0000250|UniProtKB:Q8VEK3, ECO:0000269|PubMed:11003645, ECO:0000269|PubMed:14608463, ECO:0000269|PubMed:15563465, ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:19617346, ECO:0000269|PubMed:21242313, ECO:0000269|PubMed:25986610, ECO:0000269|PubMed:9353307}
• Tissue Location: Widely expressed
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

WB (Western Blot)

(Western blot detection of hnRNP U in K562,Jurkat and Hela cell lysates and using hnRNP U mouse mAb(1:1000 diluted).Predicted band size: 110KDa.Observed band size: 110KDa.)

Related Product Information for anti-HNRNPU antibody

Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single- stranded DNA and RNA. Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription (By similarity).

References

Kiledjian M.,et al.EMBO J. 11:2655-2664(1992). Fackelmayer F.O.,et al.Biochim. Biophys. Acta 1217:232-234(1994). Fackelmayer F.O.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases. Gregory S.G.,et al.Nature 441:315-321(2006). Jordan P.,et al.Biochemistry 33:14696-14706(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 14141161
• NCBI GeneID: 3192
• NCBI Accession #: NP_004492.2
• NCBI GenBank Nucleotide #: NM_004501.3
• UniProt Accession #: Q00839; O75507; Q8N174; Q96HY9; Q9BQ09
• Molecular Weight: 110 KDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein U isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• NCBI Official Symbol: HNRNPU
• NCBI Official Synonym Symbols: SAFA; HNRPU; SAF-A; U21.1; hnRNP U; HNRNPU-AS1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein U
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein U
• UniProt Gene Name: HNRNPU
• UniProt Synonym Gene Names: HNRPU; SAFA; U21.1; hnRNP U; SAF-A
• UniProt Entry Name: HNRPU_HUMAN