Mouse anti-Human, Mouse HP1 alpha Monoclonal Antibody | anti-CBX5 antibody

HP1 alpha Antibody

Cat. Num. AAA290471

• Gene Names: CBX5; HP1; HP1A; HEL25
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Synonyms: HP1 alpha, Antibody; HP1 alpha Antibody; Purified Mouse Monoclonal Antibody (Mab); Antigen p25; Cbx 5; Cbx5; CBX5_HUMAN; CG8409; Chromobox homolog 5 (Drosophila HP1 alpha); Chromobox homolog 5 (HP1 alpha homolog Drosophila); Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Chromobox protein homologue 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP 1; HP 1A; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha; Su(var)205.; anti-CBX5 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 191

• Applicable Applications for anti-CBX5 antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).)

ICC (Immunocytochemistry)

(Immunocytochemistry stain of Hela using HP1 alpha mouse mAb (1:300).)

WB (Western Blot)

(Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa)

Related Product Information for anti-CBX5 antibody

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

References

Saunders W.S.,et al.J. Cell Sci. 104:573-582(1993). Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Ye Q.,et al.J. Biol. Chem. 271:14653-14656(1996). Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.

NCBI and Uniprot Product Information

• NCBI GI #: 188035908
• NCBI GeneID: 23468
• NCBI Accession #: NP_001120793.1
• NCBI GenBank Nucleotide #: NM_001127321.1
• UniProt Accession #: P45973; B2R8T9
• Molecular Weight: 22225
• NCBI Official Full Name: chromobox protein homolog 5
• NCBI Official Synonym Full Names: chromobox 5
• NCBI Official Symbol: CBX5
• NCBI Official Synonym Symbols: HP1; HP1A; HEL25
• NCBI Protein Information: chromobox protein homolog 5
• UniProt Protein Name: Chromobox protein homolog 5
• UniProt Gene Name: CBX5
• UniProt Synonym Gene Names: HP1A; HP1 alpha
• UniProt Entry Name: CBX5_HUMAN