Rabbit anti-Human HP1 Alpha/CBX5 Polyclonal Antibody | anti-HP1Alpha/CBX5 antibody

Anti-HP1 Alpha/CBX5 Antibody, Rabbit Polyclonal

Cat. Num. AAA258973

• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: HP1 Alpha/CBX5, Antibody; Anti-HP1 Alpha/CBX5 Antibody, Rabbit Polyclonal; chromobox homolog 5; Anti-HEL25 Antibody; Anti-HP1 Antibody; Anti-HP1A Antibody; anti-HP1Alpha/CBX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HP1 Alpha/CBX5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-HP1Alpha/CBX5 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: E coli-derived Human HP1 Alpha/CBX5 fragment
• Preparation: Produced in rabbits immunized with E coli-derived Human HP1 Alpha/CBX5 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CBX5 was immunoprecipitated using:Lane A:0.5 mg HeLa A431 Whole Cell Lysate4 uL anti-CBX5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CBX5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :22 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human CBX5 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human CBX5 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-CBX5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:24 kDa)

Related Product Information for anti-HP1Alpha/CBX5 antibody

Chromobox 5 (CBX5) is a putative target of CDC7 and genetic silencing of CBX5 also results in increased Endo180 and collagen uptake. Finally, CRISPR-mediated activation of Endo180 expression results in increased collagen uptake, suggesting that CDC7 regulates collagen internalization through increased Endo180 expression. Targeting the regulatory elements of the collagen degradative machinery may be a useful therapeutic approach in diseases of fibrosis or malignancy. The three members of the human heterochromatin protein 1 (HP1) family of proteins, HP1alpha, HP1beta, and HPgamma, are involved in chromatin packing and epigenetic gene regulation. HP1alpha is encoded from the CBX5 gene and is a suppressor of metastasis. CBX5 is down-regulated at the transcriptional and protein level in metastatic compared to non-metastatic breast cancer. CBX5 shares a bi-directional promoter structure with the hnRNPA1 gene. But whereas CBX5 expression is down-regulated in metastatic cells, hnRNAP1 expression is constant. Heterochromatin protein 1alpha (HP1alpha) encoded from the CBX5-gene is an evolutionarily conserved protein that binds histone H3 di- or tri-methylated at position lysine 9 (H3K9me2/3), a hallmark for heterochromatin, and has an essential role in forming higher order chromatin structures. HP1alpha has diverse functions in heterochromatin formation, gene regulation, and mitotic progression, and forms complex networks of gene, RNA, and protein interactions. Emerging evidence has shown that HP1alpha serves a unique biological role in breast cancer related processes and in particular for epigenetic control mechanisms involved in aberrant cell proliferation and metastasis.