Rat HSC70/HSP73 Monoclonal Antibody | anti-Heat shock protein 70; Hsc70; Hsp73 antibody

HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate)

Cat. Num. AAA76985

• Gene Names: Hspa8; HSC70
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein G affinity purified.
• Synonyms: HSC70/HSP73, Antibody; HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate); HSC70/HSP73; anti-Heat shock protein 70; Hsc70; Hsp73 antibody

• Host: Rat
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1B5
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, guinea pig, hamster, monkey, pig, rabbit and sheep HSC70 (HSP73). Detects a band of ~73kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
• Sequence Length: 646

• Applicable Applications for anti-Heat shock protein 70; Hsc70; Hsp73 antibody: EM (Electron Microscopy), WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Native hamster Hsc70 (Hsp73).
• Source: Purified from ascites.
• Preparation and Storage: Long term storage: -20 degree C; Short term storage: 4 degree C; Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of HSC70/HSP73, mAb (1B5) (biotin conjugate) : Lane 1: HSC70/HSP73 (bovine), (rec) ; Lane 2: HSP70/HSP72 (human), (recombinant) ; Lane 3: 3T3 (heat shocked) ; Lane 4: PC-12 (heat shocked) ; Lane 5: CHO-K1 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked) ; Lane 8: Vero (heat shocked).)

Related Product Information for anti-Heat shock protein 70; Hsc70; Hsp73 antibody

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Categories/Family for anti-Heat shock protein 70; Hsc70; Hsp73 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 123647
• NCBI GeneID: 100689472
• UniProt Accession #: P19378
• NCBI Official Full Name: Heat shock cognate 71 kDa protein
• NCBI Official Symbol: Hspa8
• NCBI Official Synonym Symbols: HSC70
• NCBI Protein Information: heat shock cognate 71 kDa protein
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70
• UniProt Entry Name: HSP7C_CRIGR