hSET1 recombinant antibody

Rabbit anti-hSET1 Recombinant Monoclonal Antibody [BLR024E]

Cat. Num. AAA23799

• Gene Names: SETD1A; Set1; KMT2F; Set1A
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Purified
• Synonyms: hSET1, Recombinant Antibody; Rabbit anti-hSET1 Recombinant Monoclonal Antibody [BLR024E]; Set1A; histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1; set1/Ash2 histone methyltransferase complex subunit SET1; Histone-lysine N-methyltransferase SETD1A; SET domain containing 1A; hSET1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR024E
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 10ug/ml (varies by lot)

• Applicable Applications for hSET1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:25-1:250. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF: 1:25 to 1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Immunogen: Between 1200 and 1250
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse hSET1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [AAA23799 hSET1] (AAA23799 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of human hSET1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, U2OS, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [AAA23799 hSET1] (AAA23799 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation)

(Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-hSET1 recombinant monoclonal antibody [BL-178-12C7] (AAA23799 lot 2) used for IP at 20 ul/mg lysate. hSET1 was also immunoprecipitated by a previous lot of this antibody (AAA23799 lot 1) and a second antibody against a different epitope of hSET1 . For blotting immunoprecipitated hSET1, AAA23799 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799) used at 1:25. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)

ICC (Immunocytochemistry)

(Detection of human hSET1 by immunocytochemistry. Sample: FFPE section of human jurkat cells. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)

Related Product Information for hSET1 recombinant antibody

hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain identified in drosophila proteins. hSET1 associates with a multitude of proteins in a Set1-like complex which includes hSet1, Ash2L, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, and HCF1.

Product Categories/Family for hSET1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 55741677
• NCBI GeneID: 9739
• NCBI Accession #: NP_055527.1
• NCBI GenBank Nucleotide #: NM_014712.1
• UniProt Accession #: O15047; Q6PIF3; Q8TAJ6; A6NP62
• Molecular Weight: 186,034 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETD1A
• NCBI Official Synonym Full Names: SET domain containing 1A
• NCBI Official Symbol: SETD1A
• NCBI Official Synonym Symbols: Set1; KMT2F; Set1A
• NCBI Protein Information: histone-lysine N-methyltransferase SETD1A; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD1A
• UniProt Gene Name: SETD1A
• UniProt Synonym Gene Names: hSET1A