Rabbit Hsp90 alpha/beta Monoclonal Antibody | anti-Hsp90alpha/beta antibody

Recombinant Hsp90 alpha/beta Monoclonal Antibody

Cat. Num. AAA178855

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: Hsp90 alpha/beta, Antibody; Recombinant Hsp90 alpha/beta Monoclonal Antibody; HSP84; HSPC2; HSPCB; D6S182; HSP90B; anti-Hsp90alpha/beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: R08-8A1
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
• Concentration: 300ug/mL (varies by lot)

• Applicable Applications for anti-Hsp90alpha/beta antibody: IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human Hsp90 beta
• Preparation and Storage: Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence of Hsp90 alpha/beta(green) in Hela cells using Hsp90 alpha/beta Rabbit mAb at dilution 1:200, and DAPI(blue))

IHC (Immunohiostchemistry)

(Immunohistochemistry of Hsp9 alpha/beta in paraffin-embedded Human tonsil using Hsp9 alpha/beta Rabbit mAb at dilution 1:50)

WB (Western Blot)

(Western blot detection of Hsp90 alpha/beta in Rat Brain,C6,3T3,Hela cell lysates using Hsp90 alpha/beta Rabbit mAb(1:1000 diluted).Predicted band size:85,83kDa.Observed band size:90kDa.)

Related Product Information for anti-Hsp90alpha/beta antibody

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone that is involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823).

Product Categories/Family for anti-Hsp90alpha/beta antibody

Antibodies; Primary Antibodies; Recombinant Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 153792590
• NCBI GeneID: 3320
• NCBI Accession #: NP_001017963.2
• NCBI GenBank Nucleotide #: NM_001017963.2
• UniProt Accession #: P07900; P08238
• Molecular Weight: 84,660 Da
• NCBI Official Full Name: heat shock protein HSP 90-alpha isoform 1
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha (cytosolic), class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
• NCBI Protein Information: heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; epididymis luminal secretory protein 52; heat shock
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86
• UniProt Entry Name: HS90A_HUMAN