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WB (Western Blot)
(Detection of human ICOS by western blot. Samples: Whole cell lysate (10 ug) from My-La CD4+, MOLT-4, MJ, SU-DHL-1, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
ICOS recombinant antibody
Rabbit anti-ICOS Recombinant Monoclonal Antibody [BLR194J]
Gene Names
ICOS; AILIM; CD278; CVID1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
ICOS, Recombinant Antibody; Rabbit anti-ICOS Recombinant Monoclonal Antibody [BLR194J]; inducible T-cell co-stimulator; inducible T-cell costimulator; inducible costimulator; CVID1; CD278; CD antigen CD278; activation-inducible lymphocyte immunomediatory molecule; AILIM; Inducible T-cell costimulator; inducible T cell costimulator; ICOS recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR194J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for ICOS recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 149 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ICOS by western blot. Samples: Whole cell lysate (10 ug) from My-La CD4+, MOLT-4, MJ, SU-DHL-1, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human ICOS by western blot. Samples: Whole cell lysate (10 ug) from My-La CD4+, MOLT-4, MJ, SU-DHL-1, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human ICOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from My-La CD4+ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1) used for IP at 20 ul/mg lysate. ICOS was also immunoprecipitated by a second antibody against a different epitope of ICOS (BL-1149-1E7). For blotting immunoprecipitated ICOS, AAA213635 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ICOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from My-La CD4+ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1) used for IP at 20 ul/mg lysate. ICOS was also immunoprecipitated by a second antibody against a different epitope of ICOS (BL-1149-1E7). For blotting immunoprecipitated ICOS, AAA213635 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human ICOS by immunohistochemistry. Sample: FFPE section of skin squamous cell carcinoma. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human ICOS by immunohistochemistry. Sample: FFPE section of skin squamous cell carcinoma. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of human ICOS by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of human ICOS by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human ICOS by immunocytochemistry. Sample: FFPE section of My-La CD4+ cells. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human ICOS by immunocytochemistry. Sample: FFPE section of My-La CD4+ cells. Antibody: Rabbit anti-ICOS recombinant monoclonal antibody (AAA213635 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for ICOS recombinant antibody
ICOS is a member of the CD28 and CTLA-4 cell-surface receptor family. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. ICOS is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y6W8].
Product Categories/Family for ICOS recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,127 Da
NCBI Official Full Name
inducible T-cell costimulator
NCBI Official Synonym Full Names
inducible T cell costimulator
NCBI Official Symbol
ICOS
NCBI Official Synonym Symbols
AILIM; CD278; CVID1
NCBI Protein Information
inducible T-cell costimulator
UniProt Protein Name
Inducible T-cell costimulator
UniProt Gene Name
ICOS
UniProt Synonym Gene Names
AILIM
UniProt Entry Name
ICOS_HUMAN
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Product Notes
The ICOS icos (Catalog #AAA213635) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ICOS Recombinant Monoclonal Antibody [BLR194J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICOS can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ICOS icos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICOS, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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