IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit pAb (AAA36864) at dilution of 1:100 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
Rabbit ICOS Polyclonal Antibody | anti-ICOS antibody
ICOS Polyclonal Antibody
Gene Names
ICOS; AILIM; CD278; CVID1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Purification
Synonyms
ICOS, Antibody; ICOS Polyclonal Antibody; AILIM; CD278; CVID1; ICOS/CD278; anti-ICOS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
199
Applicable Applications for anti-ICOS antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-140 of human ICOS/CD278 (NP_036224.1).
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit pAb (AAA36864) at dilution of 1:100 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit pAb (AAA36864) at dilution of 1:100 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HL-60 cells using ICOS/CD278 Rabbit pAb (AAA36864) at dilution of 1:100 (40x lens).Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HL-60 cells using ICOS/CD278 Rabbit pAb (AAA36864) at dilution of 1:100 (40x lens).Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of various lysates using ICOS/CD278 Rabbit pAb (AAA36864) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 180s)
WB (Western Blot)
(Western blot analysis of various lysates using ICOS/CD278 Rabbit pAb (AAA36864) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 180s)
Related Product Information for anti-ICOS antibody
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Product Categories/Family for anti-ICOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 30-40 kDa
Calculated MW: 23 kDa
Calculated MW: 23 kDa
NCBI Official Full Name
Inducible T-cell costimulator
NCBI Official Synonym Full Names
inducible T-cell co-stimulator
NCBI Official Symbol
ICOS
NCBI Official Synonym Symbols
AILIM; CD278; CVID1
NCBI Protein Information
inducible T-cell costimulator; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule
UniProt Protein Name
Inducible T-cell costimulator
UniProt Gene Name
ICOS
UniProt Synonym Gene Names
AILIM
UniProt Entry Name
ICOS_HUMAN
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Product Notes
The ICOS icos (Catalog #AAA36864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ICOS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ICOS can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ICOS icos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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