FCM/FACS (Flow Cytometry)
(The graph shows B-B1 with non-activated lymphocytes. The dotted line represents the IgG1 isotype control.)
Mouse anti-Human IFN-g Monoclonal Antibody | anti-IFN-g antibody
Mouse Anti-Human IFN-g Monoclonal Antibody, Azide Free Clone B-B1
Gene Names
Ifng; Ifg; IFN-g
Reactivity
Human
Applications
Functional Assay, ELISA, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
IFN-g, Antibody; Mouse Anti-Human IFN-g Monoclonal Antibody, Azide Free Clone B-B1; IFN-g; anti-IFN-g antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 Kappa light chain
Clone Number
B-B1
Specificity
Anti-Human IFN-g
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate-buffered saline. Sterile-filtered through 0.22 um. Carrier and preservative free
Concentration
1.0mg/mL (varies by lot)
Sequence Length
155
Applicable Applications for anti-IFN-g antibody
FA (Functional Assay), ELISpot, ELISA, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Production Method
Hybridoma culture supernatant (Serum free)
Appearance
Clear colorless solution
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunogen
Recombinant human IFN-g
Biological Activity
Inhibits IFNγ induced proliferation
Conjugation
Azide free
Preparation and Storage
Stable at +2-8°C short term. For longer storage freeze aliquots.
FCM/FACS (Flow Cytometry)
(The graph shows B-B1 with non-activated lymphocytes. The dotted line represents the IgG1 isotype control.)
FCM/FACS (Flow Cytometry)
(The graph shows B-B1 with non-activated lymphocytes. The dotted line represents the IgG1 isotype control.)
FCM/FACS (Flow Cytometry)
(The graph represents activated lymphocytes marked with B-B1. The dotted line represents the IgG1 isotype control.)
FCM/FACS (Flow Cytometry)
(The graph represents activated lymphocytes marked with B-B1. The dotted line represents the IgG1 isotype control.)
Related Product Information for anti-IFN-g antibody
Background: Interferon-gamma (IFN-γ, IFN-g, IFN gamma), also known as type II or immune interferon, is a dimerized soluble cytokine.
Interferon gamma is produced mainly by activated T cells and Natural Killer cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFN gamma is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s
IFNg is considered to be a proinflammatory cytokine, that activates macrophages and endothelial cells, it also regulates immune responses by effecting APC, and T cells.
IFN gamma is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFN gamma is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects.
Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFN is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.
IFN is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFN? during an immune response will result in the preferential proliferation of Th1 cells.
In addition, IFNγ has several properties related to immunoregulation. IFN? is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFγ. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.
The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.
Interferon gamma is produced mainly by activated T cells and Natural Killer cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFN gamma is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s
IFNg is considered to be a proinflammatory cytokine, that activates macrophages and endothelial cells, it also regulates immune responses by effecting APC, and T cells.
IFN gamma is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFN gamma is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects.
Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFN is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.
IFN is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFN? during an immune response will result in the preferential proliferation of Th1 cells.
In addition, IFNγ has several properties related to immunoregulation. IFN? is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFγ. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.
The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.
References
Aldemir et.al J immunol 2005
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
Ifng
NCBI Official Synonym Symbols
Ifg; IFN-g
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
Ifng
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_MOUSE
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Product Notes
The IFN-g ifng (Catalog #AAA58558) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IFN-g Monoclonal Antibody, Azide Free Clone B-B1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFN-g can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), ELISpot, ELISA, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IFN-g ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-g, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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