Rabbit anti-Human IGF1R Monoclonal Antibody | anti-IGF1R antibody

Recombinant Anti-IGF1R Antibody, Rabbit Monoclonal

Cat. Num. AAA254609

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: IGF1R, Antibody; Recombinant Anti-IGF1R Antibody, Rabbit Monoclonal; IGF1R/CD221 Antibody, Rabbit MAb; insulin-like growth factor 1 receptor; Anti-CD221 Antibody; Anti-IGF-I R Antibody; Anti-IGF1 Receptor Antibody; Anti-IGFIR Antibody; Anti-IGFR Antibody; Anti-JTK13 Antibody; anti-IGF1R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 012
• Specificity: Human IGF1R/CD221
• Purity/Purification: Protein A
• Form/Format: 0.2um filtered solution in PBS

• Applicable Applications for anti-IGF1R antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human IGF1R/CD221 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human IGF1R/CD221 (rh IGF1R/CD221).
• Preparation and Storage: This antibody can be stored at 2-°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.Flow cytometry was performed on a BD FACSCalibur flow cytometry system.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)

Related Product Information for anti-IGF1R antibody

The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits, and among which, the alpha subunit is extracellular while the beta subunit has an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. The IGF1R signaling pathway is activated in the mammalian nervous system from the early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signaling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumors and mediates proliferation and apoptosis protection. IGF signaling also influences hypoxia signaling, protease secretion, tumor cell motility, and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, IGF1R is now an attractive anti-cancer treatment target.

Product Categories/Family for anti-IGF1R antibody

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets; AKT Signaling Pathway

References

Bhr C, et al. (2004) The insulin like growth factor-1 receptor (IGF-1R) as a drug target: novel approaches to cancer therapy. Growth Horm IGF Res. 14 (4): 287-95.
Riedemann J, et al. (2006) IGF1R signalling and its inhibition. Endocr Relat Cancer. 13 Suppl 1: 33-43.
Gualco E, et al. (2009) IGF-IR in neuroprotection and brain tumors. Front Biosci. 14: 352-75.
Annenkov A. (2009) The insulin-like growth factor (IGF) receptor type 1 (IGF1R) as an essential component of the signalling network regulating neurogenesis. Mol Neurobiol. 40 (3): 195-215.

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.3
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_HUMAN