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product-image-AAA259473_WB13.png WB (Western Blot) (Anti-IL-3R alpha/CD123 monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human IL-3R alpha/CD123 Protein 100ngSecondaryRabbit Anti-Mouse IgG2b/HRP(#AAA259473-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment))

IgG2b Recombinant Antibody | Ighg2b recombinant antibody

Rabbit Anti-Mouse IgG2b Secondary Antibody, Rabbit MAb

Reactivity
Mouse
Applications
ELISA
Purity
Protein A
Synonyms
IgG2b, Recombinant Antibody; Rabbit Anti-Mouse IgG2b Secondary Antibody, Rabbit MAb; Anti-gamma2b Antibody; Anti-IgG2b Antibody; Anti-Igh-3 Antibody; Ighg2b recombinant antibody
Ordering
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
039
Specificity
This antibody recognizes mouse IgG2b, but almost not reacts to Mouse IgG1, IgG2a, IgG3, IgM.
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for Ighg2b recombinant antibody
ELISA
Immunogen
Recombinant Mouse IgG2b-Fc Protein ( P01867-2; Glu97-Lys335).
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Anti-IL-3R alpha/CD123 monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human IL-3R alpha/CD123 Protein 100ngSecondaryRabbit Anti-Mouse IgG2b/HRP(#AAA259473-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment))

product-image-AAA259473_WB13.png WB (Western Blot) (Anti-IL-3R alpha/CD123 monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human IL-3R alpha/CD123 Protein 100ngSecondaryRabbit Anti-Mouse IgG2b/HRP(#AAA259473-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment))

ELISA

(ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG2b Secondary Antibody (#AAA259473) reacts only to mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM. The plate was coated with 0.1ug/mL of different mouse immunoglobulins. 0.1ug/mL of Rabbit Anti-Mouse IgG2b Secondary Antibody (#AAA259473) was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody.)

product-image-AAA259473_ELISA15.png ELISA (ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG2b Secondary Antibody (#AAA259473) reacts only to mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM. The plate was coated with 0.1ug/mL of different mouse immunoglobulins. 0.1ug/mL of Rabbit Anti-Mouse IgG2b Secondary Antibody (#AAA259473) was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody.)

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
36,588 Da
NCBI Official Full Name
Ig gamma-2B chain C region, partial
UniProt Protein Name
Ig gamma-2B chain C region
UniProt Gene Name
Igh-3
UniProt Entry Name
IGG2B_MOUSE

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Product Notes

The Ighg2b igh-3 (Catalog #AAA259473) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Mouse IgG2b Secondary Antibody, Rabbit MAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2b can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the Ighg2b igh-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2b, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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