Mouse IgY Monoclonal Antibody | anti-IgY antibody

MOUSE ANTI DUCK IgY HEAVY CHAIN

Cat. Num. AAA49384

• Reactivity: Goose; Does not react with Chicken; N.B. Antibody reactivity and working conditions may vary between species.
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Synonyms: IgY, Antibody; MOUSE ANTI DUCK IgY HEAVY CHAIN; anti-IgY antibody

• Host: Mouse
• Reactivity: Goose; Does not react with Chicken; N.B. Antibody reactivity and working conditions may vary between species.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 16C7
• Specificity: IgY Heavy Chain
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)

• Applicable Applications for anti-IgY antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Purified Pekin duck yolk IgY.
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
• Buffer Solution: Phosphate buffered saline
• Target Species: Duck
• Specificity Note: MBS212832 recognizes both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab’)2 fragment of IgY.; MBS212832 also detects IgY heavy chain in Mallard ducks, goose and swan.; MBS212832 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-IgY antibody

MBS212832 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab')2 fragment of IgY. MBS212832 also detects IgY heavy chain in Mallard ducks, goose and swan. MBS212832 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.