WB (Western Blot)
(The anti-IKKbeta Mab is used in Western blot to detect IKKbeta in Jurkat cell lysate.)
Mouse anti-Human IKK beta Monoclonal Antibody | anti-IKBKB antibody
IKK beta Antibody
Gene Names
IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
IKK beta, Antibody; IKK beta Antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB; anti-IKBKB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
62AT216
Specificity
This IKK beta antibody was raised using purified His-tagged recombinant full length human IKK beta.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
fomat Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.65 (varies by lot)
Sequence Length
697
Applicable Applications for anti-IKBKB antibody
ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(The anti-IKKbeta Mab is used in Western blot to detect IKKbeta in Jurkat cell lysate.)
WB (Western Blot)
(The anti-IKKbeta Mab is used in Western blot to detect IKKbeta in Jurkat cell lysate.)
WB (Western Blot)
(All lanes : Anti-IKK beta Antibody at 1:4000 dilutionLane 1: HL-60 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 87 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-IKK beta Antibody at 1:4000 dilutionLane 1: HL-60 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 87 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-IKBKB antibody
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Product Categories/Family for anti-IKBKB antibody
References
Downregulation of active IKK beta by Ro52-mediated autophagy. Niida M, et al. Mol Immunol, 2010 Aug. PMID 20627395.
Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. Schuurhof A, et al. Pediatr Pulmonol, 2010 Jun. PMID 20503287.
Association between anti-tumour necrosis factor treatment response and genetic variants within the TLR and NF{kappa}B signalling pathways. Potter C, et al. Ann Rheum Dis, 2010 Jul. PMID 20448286.
Protein phosphatase 2A acts as a mitogen-activated protein kinase kinase kinase 3 (MEKK3) phosphatase to inhibit lysophosphatidic acid-induced IkappaB kinase beta/nuclear factor-kappaB activation. Sun W, et al. J Biol Chem, 2010 Jul 9. PMID 20448038.
Respiratory syncytial virus-mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I, TRAF6, and IKK beta. Yoboua F, et al. J Virol, 2010 Jul. PMID 20410276.
Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. Schuurhof A, et al. Pediatr Pulmonol, 2010 Jun. PMID 20503287.
Association between anti-tumour necrosis factor treatment response and genetic variants within the TLR and NF{kappa}B signalling pathways. Potter C, et al. Ann Rheum Dis, 2010 Jul. PMID 20448286.
Protein phosphatase 2A acts as a mitogen-activated protein kinase kinase kinase 3 (MEKK3) phosphatase to inhibit lysophosphatidic acid-induced IkappaB kinase beta/nuclear factor-kappaB activation. Sun W, et al. J Biol Chem, 2010 Jul 9. PMID 20448038.
Respiratory syncytial virus-mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I, TRAF6, and IKK beta. Yoboua F, et al. J Virol, 2010 Jul. PMID 20410276.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86564
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
NCBI Official Synonym Full Names
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
NCBI Official Symbol
IKBKB
NCBI Official Synonym Symbols
IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Gene Name
IKBKB
UniProt Synonym Gene Names
IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
UniProt Entry Name
IKKB_HUMAN
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Product Notes
The IKBKB ikbkb (Catalog #AAA283428) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IKK beta Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IKK beta can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IKBKB ikbkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKK beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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