Rabbit anti-Human IKK-beta Polyclonal Antibody | anti-IKK-beta antibody

Rabbit anti-IKK-beta Antibody, Affinity Purified

Cat. Num. AAA211646

• Gene Names: IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: IKK-beta, Antibody; Rabbit anti-IKK-beta Antibody, Affinity Purified; IKK-beta; inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; IMD15; IKKB; IKK2; IkBKB; nuclear factor NF-kappa-B inhibitor kinase beta; I-kappa-B-kinase beta; I-kappa-B kinase 2; IKK-B; Inhibitor of nuclear factor kappa-B kinase subunit beta; inhibitor of nuclear factor kappa B kinase subunit beta; anti-IKK-beta antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-IKK-beta antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 706 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human IKK-beta by western blot. Samples: Whole cell lysate from HeLa (H; 50 ug), Jurkat (J; 50 ug), MCF-7 (M; 50 ug), and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-IKK-beta antibody AAA211646 (lot AAA211646-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3 minute.)

IP (Immunoprecipitation)

(Detection of human IKK-beta by western blot of immunoprecipitatesion. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-IKK-beta antibody AAA211646 (lot AAA211646-1) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated IKK-beta, AAA211646 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

Related Product Information for anti-IKK-beta antibody

IKK-beta (Inhibitor of nuclear factor kappa B kinase subunit beta) is one of three subunits that comprise the IKK complex. The IKK complex consists of two catalytic subunits, IKK-alpha and IKK-beta, and a regulatory subunit IKK-gamma/NEMO. As a kinase, IKK functions to phosphorylate members of the NF-kappa B inhibitor proteins, the I-kappa B proteins. Phosphorylation of I-kappa B proteins results in the ubiquitination and degradation of I kappa B and the release and translocation of the NF-kappa B transcription factor to the nucleus where it may function to activate a vast number of genes.

Product Categories/Family for anti-IKK-beta antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 41352712
• NCBI GeneID: 3551
• NCBI Accession #: NP_001547.1
• NCBI GenBank Nucleotide #: NM_001556.2
• UniProt Accession #: O14920; O75327; B4DZ30; B4E0U4
• Molecular Weight: 86,564 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 1
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN