Mouse anti-Human IL-33 Monoclonal Antibody | anti-IL-33 antibody

Anti-IL-33 Antibody, Mouse Monoclonal

Cat. Num. AAA258795

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A
• Synonyms: IL-33, Antibody; Anti-IL-33 Antibody, Mouse Monoclonal; interleukin 33; Anti-C9orf26 Antibody; Anti-DVS27 Antibody; Anti-IL1F11 Antibody; Anti-NF-HEV Antibody; Anti-NFEHEV Antibody; anti-IL-33 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: #50
• Specificity: Human IL-33
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL-33 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Recombinant Human IL-33 Protein
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(IL-33 was immunoprecipitated using:Lane A:0.5 mg HeLa, K562, jurkat Whole Cell Lysate2 uL anti-IL-33 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IL-33 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size :25 kDa)

WB (Western Blot)

(Anti-IL-33 mouse monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:25 kDa)

Related Product Information for anti-IL-33 antibody

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

References

Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.