FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-IL-9R antibody AH9R2 . Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human IL-9R Monoclonal Antibody | anti-IL-9R antibody
Anti-IL-9R [AH9R2]
Gene Names
IL9R; CD129; IL-9R
Reactivity
Human
Applications
Immunoprecipitation
Purity
Purified antibody. Protein A affinity purified
Synonyms
IL-9R, Antibody; Anti-IL-9R [AH9R2]; Interleukin-9 receptor; IL9R; CD129; anti-IL-9R antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a, kappa
Clone Number
AH9R2
Specificity
This antibody binds specifically to the human IL-9 receptor alpha chain.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-9R antibody
Blocking, IP (Immunoprecipitation)
Chimeric Use Statement
This reformatted antibody was made using the variable domain sequences of the original mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
PMID
8756628
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-IL-9R antibody AH9R2 . Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-IL-9R antibody AH9R2 . Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-IL-9R antibody
Recombinant monoclonal antibody to IL-9R. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AH9R2.
This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.
This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.
Product Categories/Family for anti-IL-9R antibody
References
Demoulin et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996 Sep;16(9):4710-6.
Note on publication: Describes the original generation of this antibody, and its use in immunoprecipitation analysis.
Note on publication: Describes the original generation of this antibody, and its use in immunoprecipitation analysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,147 Da
NCBI Official Full Name
interleukin-9 receptor isoform 1
NCBI Official Synonym Full Names
interleukin 9 receptor
NCBI Official Symbol
IL9R
NCBI Official Synonym Symbols
CD129; IL-9R
NCBI Protein Information
interleukin-9 receptor; IL-9 receptor
UniProt Protein Name
Interleukin-9 receptor
UniProt Gene Name
IL9R
UniProt Synonym Gene Names
IL-9 receptor; IL-9R
UniProt Entry Name
IL9R_HUMAN
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Product Notes
The IL-9R il9r (Catalog #AAA72119) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-9R [AH9R2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-9R can be used in a range of immunoassay formats including, but not limited to, Blocking, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the IL-9R il9r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-9R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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