Rabbit anti-Human IL3RA Monoclonal Antibody | anti-IL3Ra antibody

Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal

Cat. Num. AAA254986

• Gene Names: IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A
• Synonyms: IL3RA, Antibody; Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal; IL3RA/CD123 Antibody, Rabbit MAb; interleukin 3 receptor, alpha (low affinity); Anti-CD123 Antibody; Anti-hIL-3Ra Antibody; Anti-IL3R Antibody; Anti-IL3RAY Antibody; Anti-IL3RX Antibody; Anti-IL3RY Antibody; anti-IL3Ra antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 017
• Specificity: Human IL3RA/CD123.; No cross-reactivity in ELISA with Human IL6R
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL3Ra antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human IL3RA protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human IL3RA/CD123 (rh L3RA).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(IL3RA was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-IL3RA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IL3RA rabbit monoclonal antibody,at 1:350 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 38 kDa)

WB (Western Blot)

(Anti-IL3RA rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:38 kDa)

Related Product Information for anti-IL3Ra antibody

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and/or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

Product Categories/Family for anti-IL3Ra antibody

AKT Signaling Pathway

References

Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.

NCBI and Uniprot Product Information

• NCBI GI #: 417184
• NCBI GeneID: 3563
• UniProt Accession #: P26951; Q5HYQ7; Q5HYQ8; Q9UEH7; A8K3F3; B9VI81
• Molecular Weight: Observed MW: 70kDa; Calculated MW: 34kDa/43kDa
• NCBI Official Full Name: Interleukin-3 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 3 receptor, alpha (low affinity)
• NCBI Official Symbol: IL3RA
• NCBI Official Synonym Symbols: IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• NCBI Protein Information: interleukin-3 receptor subunit alpha; IL-3RA; IL-3R-alpha; CD123 antigen; IL-3R subunit alpha; IL-3 receptor subunit alpha; IL-3 receptor alpha SP2 isoform
• UniProt Protein Name: Interleukin-3 receptor subunit alpha
• UniProt Gene Name: IL3RA
• UniProt Synonym Gene Names: IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
• UniProt Entry Name: IL3RA_HUMAN