WB (Western Blot)
(Western Blot: The cell lysate derived from Human IM-9 lymphocytes (5 mg/ml) was immunoprecipitated by Mouse anti IR-1 (Cat# AAA71317) at 10 ug/ml. A major band around ~135 kDa is observed.)
Mouse anti-Human Insulin Receptor (IR) Monoclonal Antibody | anti-IR antibody
Mouse anti Insulin Receptor (IR) Monoclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Purified by Protein A-Affinity Chromatography according to Isotyping
Synonyms
Insulin Receptor (IR), Antibody; Mouse anti Insulin Receptor (IR) Monoclonal Antibody; Insulin receptor; CD220; IR; anti-IR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
ABM258
Specificity
This antibody recognizes human Insulin Receptor protein. The other species was not tested.
Purity/Purification
Purified by Protein A-Affinity Chromatography according to Isotyping
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-IR antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Preparation
Highly purified human placental Insulin Receptor.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C. The antibodies can be stored at 2°C - 80°C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(Western Blot: The cell lysate derived from Human IM-9 lymphocytes (5 mg/ml) was immunoprecipitated by Mouse anti IR-1 (Cat# AAA71317) at 10 ug/ml. A major band around ~135 kDa is observed.)
WB (Western Blot)
(Western Blot: The cell lysate derived from Human IM-9 lymphocytes (5 mg/ml) was immunoprecipitated by Mouse anti IR-1 (Cat# AAA71317) at 10 ug/ml. A major band around ~135 kDa is observed.)
Related Product Information for anti-IR antibody
The human insulin receptor is a heterotetrametric membrane glycoprotein consisting of disulfide-linked subunits in a β-α-α-β Configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha subunit (135kDa) is completely extracellular.
References
Kull FC, et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J Biol Chem. 1983 May 25;258 (10):6561-6.
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Product Notes
The IR (Catalog #AAA71317) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Insulin Receptor (IR) Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Receptor (IR) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the IR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Receptor (IR), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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