IF (Immunofluorescence)
(ICC/IF analysis of IRF5 in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human THP-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of IRF5 in Raw264.7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IRF5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human, Mouse IRF-5 Monoclonal Antibody | anti-IRF-5 antibody
IRF-5 antibody
Gene Names
IRF5; SLEB10
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
IRF-5, Antibody; IRF-5 antibody; interferon regulatory factor 5; irf5; anti-IRF-5 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
10T1
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
147
Applicable Applications for anti-IRF-5 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human IRF-5 (176-240aa) purified from E. coli
Clone Note
Anti-human IRF-5 mAb, clone 10T1, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-5 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of IRF5 in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human THP-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of IRF5 in Raw264.7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IRF5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of IRF5 in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human THP-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of IRF5 in Raw264.7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IRF5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry)
(Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
IHC (Immunohiostchemistry)
(Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
WB (Western Blot)
(The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-IRF-5 antibody
Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.
Product Categories/Family for anti-IRF-5 antibody
References
Mario B. et al. (2007) J. Virology 81 (9):4473-4481.; Ning S. et al. (2005) J. Virology 79:11671-11676.; Mario B. et al. (2007) J. Virology 81 (9):4473-4481
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,020 Da
NCBI Official Synonym Full Names
interferon regulatory factor 5
NCBI Official Symbol
IRF5
NCBI Official Synonym Symbols
SLEB10
NCBI Protein Information
interferon regulatory factor 5
UniProt Protein Name
Interferon regulatory factor 5
UniProt Gene Name
IRF5
UniProt Synonym Gene Names
IRF-5
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Product Notes
The IRF-5 irf5 (Catalog #AAA47985) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IRF-5 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRF-5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IRF-5 irf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF-5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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