Mouse anti-Human ISG15/UCRP Monoclonal Antibody | anti-ISG15/UCRP antibody

ISG15/UCRP

Cat. Num. AAA48297

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: By protein-A affinity chromatography
• Synonyms: ISG15/UCRP, Antibody; ISG15/UCRP; Interferon-stimulated gene 15 kDa ubiquitin-like modifier; GIP2; uCRP,IFI15,ISG15,Interferon-stimulated gene; 15 kDa ubiquitin-like modifier; Interferon-stimulated gene,15 kDa ubiquitin-like modifier IFI 15; Interferon alpha inducible protein; Interferon induced 15 kDaprotein; Interferon induced 17 kDa protein; Interferon induced 17 kDa/15 kDa protein; InterferonStimulated Gene 15; Interferon stimulated protein 15 kDa; ISG 15; ISG15 ubiquitin like modifier,ubiquitin cross reactive protein precursor; anti-ISG15/UCRP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 3E5
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 165

• Applicable Applications for anti-ISG15/UCRP antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human ISG15 (1-157aa) purified from E. coli
• Conjugation: Unconjugated
• Product Note: This product was produced from tissue culture supernatant.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

WB (Western Blot)

(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;Lane 2.: PC3 cell lysate;;)

ICC (Immunocytochemistry)

(ICC/IF analysis of ISG15 in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Related Product Information for anti-ISG15/UCRP antibody

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play importantroles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety ofproteins when cells are treated with type I interferon or lipopolysaccharide.

Product Categories/Family for anti-ISG15/UCRP antibody

Epigenetics and Nuclear Signaling; Immunology

References

Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215.;;Weiming Y et al.,(2001) EMBO J 20(3):362-371.;;Chen Z et al.,(2005) Proc Natl Acad Sci USA 102(29):10200-10205.;;

NCBI and Uniprot Product Information

• NCBI GI #: 4826774
• NCBI GeneID: 9636
• NCBI Accession #: NP_005092.1
• NCBI GenBank Nucleotide #: NP_005092.1
• UniProt Accession #: P05161
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP
• UniProt Entry Name: ISG15_HUMAN