FCM/FACS (Flow Cytometry)
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Rat ITGAM / CD11b Monoclonal Antibody | anti-ITGAM antibody
Rat Monoclonal [clone M1/70.15] (IgG2b) to Mouse ITGAM / CD11b
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human, Mouse, Rabbit
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunohistochemistry
Purity
Ion exchange Chromatography
Synonyms
ITGAM / CD11b, Antibody; Rat Monoclonal [clone M1/70.15] (IgG2b) to Mouse ITGAM / CD11b; Anti-ITGAM / CD11b Antibody (clone M1/70.15) IHC-plus; ITGAM; Antigen CD11b (p170); CD11B; CD11b antigen; CR3A; Integrin alpha-M; MAC-1; MO1A; Neutrophil adherence receptor; MAC1A; SLEB6; CR-3 alpha chain; Mouse ITGAM; CD11b; anti-ITGAM antibody
Host
Rat
Reactivity
Human, Mouse, Rabbit
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M1/70.15
Specificity
Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Purity/Purification
Ion exchange Chromatography
Form/Format
PBS, 0.09% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1152
Applicable Applications for anti-ITGAM antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target Species
Mouse
Immunogen Description
T cell enriched splenocytes from B10 mice.
Immunogen Type
Cells
Immunogen
ITGAM / CD11b antibody was raised against t cell enriched splenocytes from B10 mice.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
FCM/FACS (Flow Cytometry)
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
IHC (Immunohistochemistry)
(Anti-CD11b antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-CD11b antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Related Product Information for anti-ITGAM antibody
The leukocyte adhesion receptor Mac-1 is a cell surface glycoprotein that plays a fundamental role in the adhesion between myeloid and lymphoid cells. It is composed of alpha/beta heterodimers and expressed on cells of myeloid linage. CD11b constitutes the alpha subunit and is a member the integrin family of proteins. Mac-1 has been shown to be involved in neutrophil aggregation and adhesion to endothelial cells, rosetting of erythrocytes to macrophages and polymorphonuclear leukocytes.
Product Categories/Family for anti-ITGAM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
127,307 Da
NCBI Official Full Name
integrin alpha-M isoform 2
NCBI Official Synonym Full Names
integrin, alpha M (complement component 3 receptor 3 subunit)
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
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Product Notes
The ITGAM itgam (Catalog #AAA51907) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Monoclonal [clone M1/70.15] (IgG2b) to Mouse ITGAM / CD11b reacts with Human, Mouse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's ITGAM / CD11b can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGAM / CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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