Mouse anti-Human ITGB2 / MAC-1 / CD18 Monoclonal Antibody | anti-ITGB2 antibody

Mouse Monoclonal [clone MEM-48] (IgG1) to Human ITGB2 / MAC-1 / CD18

Cat. Num. AAA12341

• Gene Names: ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A affinity chromatography.; >95% by SDS-PAGE.
• Synonyms: ITGB2 / MAC-1 / CD18, Antibody; Mouse Monoclonal [clone MEM-48] (IgG1) to Human ITGB2 / MAC-1 / CD18; Anti-ITGB2 / MAC-1 / CD18 Antibody (clone MEM-48) IHC-plus; ITGB2; CD18 antigen; CD18; Integrin beta chain; beta 2; LCAMB; MAC-1; MFI7; MF17; Integrin beta-2; LAD; LFA-1; Human ITGB2; anti-ITGB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-48
• Specificity: The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Purity/Purification: Purified by Protein A affinity chromatography.; >95% by SDS-PAGE.
• Form/Format: PBS, 15 mM sodium azide, pH 7.4.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 769

• Applicable Applications for anti-ITGB2 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC), Functional Assay
• Application Notes: IHC-p: 10 ug/ml; WB: 2 ug/ml; IP: 2 ug/ml; FC: 2 ug/ml; ; Optimal dilutions/concentrations should be determined by the end user
• Immunogen: Raised against Leukocytes of a patient suffering from a LGL-type leukemia
• Target Species: Human
• Immunogen Species: ITCB2/CD18 antibody was raised against human.
• Family/Subfamily: Integrin/Not assigned-Integrin
• Antigen Type: Cells
• Non-Reactivity: Canine
• Target: ITGB2/CD18
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60 degree C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration:; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 degree C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate:; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB); Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 2 degree C to 8 degree C. Do not freeze.

IHC (Immunohistochemistry)

(Anti-ITGB2 / CD18 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-ITGB2 antibody

Family: Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 89191865
• NCBI GeneID: 3689
• NCBI Accession #: NP_000202.2
• NCBI GenBank Nucleotide #: NM_000211.3
• UniProt Accession #: P05107; Q16418; Q53HS5; Q9UD72; B3KTS8; D3DSM1
• NCBI Official Full Name: integrin beta-2
• NCBI Official Synonym Full Names: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• NCBI Official Symbol: ITGB2
• NCBI Official Synonym Symbols: LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• NCBI Protein Information: integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor)
• UniProt Protein Name: Integrin beta-2
• UniProt Gene Name: ITGB2
• UniProt Synonym Gene Names: CD18; MFI7
• UniProt Entry Name: ITB2_HUMAN