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IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
Rabbit JNK1/2/3-T183/T183/T221 Monoclonal Antibody | anti-JNK1/2/3-T183/T183/T221 antibody
Phospho-JNK1/2/3-T183/T183/T221 Monoclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
JNK1/2/3-T183/T183/T221 ; Monoclonal Antibody; Phospho-JNK1/2/3-T183/T183/T221 Monoclonal Antibody ; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; anti-JNK1/2/3-T183/T183/T221 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
427
Applicable Applications for anti-JNK1/2/3-T183/T183/T221 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Immunogen
A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HUVEC cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HUVEC cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human uterus using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human uterus using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using Phospho-JNK1/2/3-T183/T183/T221 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
WB (Western Blot)
(Western blot analysis of extracts of various cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Related Product Information for anti-JNK1/2/3-T183/T183/T221 antibody
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Product Categories/Family for anti-JNK1/2/3-T183/T183/T221 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46; 54 kDa
NCBI Official Full Name
Mitogen-activated protein kinase 8
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8
NCBI Official Symbol
MAPK8
NCBI Official Synonym Symbols
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
NCBI Protein Information
mitogen-activated protein kinase 8
UniProt Protein Name
Mitogen-activated protein kinase 8
UniProt Gene Name
MAPK8
UniProt Synonym Gene Names
JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
Similar Products
Product Notes
The JNK1/2/3-T183/T183/T221 mapk8 (Catalog #AAA28260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-JNK1/2/3-T183/T183/T221 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JNK1/2/3-T183/T183/T221 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the JNK1/2/3-T183/T183/T221 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1/2/3-T183/T183/T221 , Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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