kappa light chain Recombinant Antibody | Igkc recombinant antibody

Anti-Mouse antibody kappa light chain [OX-20], Rabbit IgG, kappa

Cat. Num. AAA72482

• Gene Names: Igkc; Igk-C
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified
• Synonyms: kappa light chain, Recombinant Antibody; Anti-Mouse antibody kappa light chain [OX-20], Rabbit IgG, kappa; Mouse antibody kappa light chain; Anti-Mouse antibody kappa light chain [OX-20]; kappa Lc; OX20; MRC OX-20; MRC OX20; Igkc recombinant antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: OX-20
• Specificity: OX-20 binds specifically to mouse kappa light chain of immunoglobulins. Kappa light chains are found in 95% of mouse Igs with lambda light chains being found in other antibodies. Kappa and lambda chains differ slightly in amino acids residue composition.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for Igkc recombinant antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

ELISA

(ELISAusingOX-20(AAA72482),Ab00102-1.1,Ab00102-2.0andAb00104-1.1. BindingcurvesoftherabbitIgGchimericversionoftheAnti-HAantibodyOX-20againstAb00102-1.1(blueline),Ab00102-2.0(orangeline)andAb00104-1.1(greyline)toanELISAplatecoatedwithAb00102-1.1,Ab00102-2.0andAb00104-1.1ataconcentrationof2.5ug/ml.A3-foldserialdilutionfrom3000to0.0169ng/mlwasperformedusingAAA72482antibody.Forsignaldetection,a1:4000dilutionofaHRP-conjugatedanti-rabbitIgGantibodywasused.)

FCM/FACS (Flow Cytometry)

(FlowcytometryusingtheAnti-MouseantibodykappalightchainantibodyOX-20(AAA72482). mousesplenocyteswerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-20(AAA72482,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)

IF (Immunofluorescence)

(Immunofluorescencestainingoffixedmousesplenocyteswithanti-MouseantibodykappalightchainantibodyOX-20(AAA72482) ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytesonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofOX-20(AAA72482)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72482,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)

WB (Western Blot)

(Western Blotusinganti-MouseantibodykappalightchainantibodyOX-20(AAA72482) Mousespleen(A)andmousethymus(B)tissuelysates(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofOX-20(AAA72482)at0.001ug/mland0.0001ug/ml,respectively,beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)

Related Product Information for Igkc recombinant antibody

OX-20 was generated by hyperimmunizing rats with mouse F(ab')2 generated by enzymatic digest.

References

Mason et al. The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies. Immunology 44:75-87 1981 N/A6456222
Note on publication:
Describes how rat anti-mouse antibodies were shown to bind specifically to IgGs and not to IgM by indirect haemagglutination of sheep red blood cells which had been coated with different mouse mAbs specific for sheep red blood cells, and to mouse IgGs and not IgG molecules derived from other species (some cross reaction with rabbit and guinea pig) (Galfre et al. 1979).

NCBI and Uniprot Product Information

• NCBI GI #: 125146
• NCBI GeneID: 16071
• UniProt Accession #: P01837
• Molecular Weight: 11,778 Da
• NCBI Official Full Name: Ig kappa chain C region, partial
• NCBI Official Synonym Full Names: immunoglobulin kappa constant
• NCBI Official Symbol: Igkc
• NCBI Official Synonym Symbols: Igk-C
• UniProt Protein Name: Ig kappa chain C region
• UniProt Entry Name: IGKC_MOUSE