IF (Immunofluorescence)
(Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Mouse anti-Human KDM5C Monoclonal Antibody | anti-KDM5C antibody
Anti-KDM5C Antibody
Gene Names
KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
KDM5C, Antibody; Anti-KDM5C Antibody; anti-KDM5C antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of KDM5C protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH7.3.
Applicable Applications for anti-KDM5C antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E coli
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of KDM5C expression in Hela (A), 293T (B), T47D (C), MCF7 (D) whole cell lysates. (Predicted band size: 176 kD; Observed band size: 220 kD))
WB (Western Blot)
(Western blot analysis of KDM5C expression in Hela (A), 293T (B), T47D (C), MCF7 (D) whole cell lysates. (Predicted band size: 176 kD; Observed band size: 220 kD))
Related Product Information for anti-KDM5C antibody
Mouse monoclonal antibody to KDM5C
Product Categories/Family for anti-KDM5C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1560
NCBI Official Full Name
Lysine-specific demethylase 5C
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5C
NCBI Official Symbol
KDM5C
NCBI Official Synonym Symbols
MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
NCBI Protein Information
lysine-specific demethylase 5C; Smcy homolog, X-linked; Smcx homolog, X chromosome; histone demethylase JARID1C; JmjC domain-containing protein SMCX; Jumonji/ARID domain-containing protein 1C; Jumonji, AT rich interactive domain 1C (RBP2-like)
UniProt Protein Name
Lysine-specific demethylase 5C
UniProt Gene Name
KDM5C
UniProt Synonym Gene Names
DXS1272E; JARID1C; SMCX; XE169
UniProt Entry Name
KDM5C_HUMAN
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Product Notes
The KDM5C kdm5c (Catalog #AAA265743) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-KDM5C Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KDM5C can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the KDM5C kdm5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM5C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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