Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
JARID1C/KDM5C recombinant protein
Recombinant JARID1C/KDM5C protein
Gene Names
KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Synonyms
JARID1C/KDM5C; N/A; Recombinant JARID1C/KDM5C protein; Lysine Demethylase 5C; Jumonji; AT Rich Interactive Domain 1C (RBP2-Like); JARID1C/KDM5C recombinant protein
Host
Baculovirus
Form/Format
Full length recombinant JARID1C/KDM5C protein is supplied at a concentration of 0.5ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant JARID1C/KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions:50mM HEPES pH 7.5, 0.02% Triton X100, 100uM 2OG, 100uM Ascorbate, 50uM (NH4)2Fe(SO4)2·6H2O, 1mM TCEP, 100nm Recombinant JARID1C/KDM5C protein, and 3.3uM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
SDS-PAGE
(Recombinant JARID1C / KDM5C protein gel. JARID1C / KDM5C protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 180 kDa Purity: > 87%)
SDS-PAGE
(Recombinant JARID1C / KDM5C protein gel. JARID1C / KDM5C protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 180 kDa Purity: > 87%)
Related Product Information for JARID1C/KDM5C recombinant protein
Short Description: Recombinant JARID1C/KDM5C (accession number NP_004178.2) was expressed in Sf9 and contains an N-terminal DYKDDDDK tag with a molecular weight of 180 kDa. Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation. Recombinant JARID1C/KDM5C can be used for enzyme kinetics and inhibitor screening studies.
Background: Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.
Background: Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.
Product Categories/Family for JARID1C/KDM5C recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
175,592 Da
NCBI Official Full Name
lysine-specific demethylase 5C isoform 1
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5C
NCBI Official Symbol
KDM5C
NCBI Official Synonym Symbols
MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
NCBI Protein Information
lysine-specific demethylase 5C; Smcy homolog, X-linked; Smcx homolog, X chromosome; histone demethylase JARID1C; JmjC domain-containing protein SMCX; Jumonji/ARID domain-containing protein 1C; Jumonji, AT rich interactive domain 1C (RBP2-like)
UniProt Protein Name
Lysine-specific demethylase 5C
UniProt Gene Name
KDM5C
UniProt Synonym Gene Names
DXS1272E; JARID1C; SMCX; XE169
UniProt Entry Name
KDM5C_HUMAN
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Product Notes
The JARID1C/KDM5C kdm5c (Catalog #AAA59681) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JARID1C/KDM5C, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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