WB (Western Blot)
(Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.)
Mouse anti-Human KEAP1 Monoclonal Antibody | anti-KEAP1 antibody
KEAP1 Antibody
Gene Names
KEAP1; INrf2; KLHL19
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affintiy purification
Synonyms
KEAP1, Antibody; KEAP1 Antibody; Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like family member 19; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; anti-KEAP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
KEAP1 antibody detects endogenous levels of total KEAP1
Purity/Purification
Peptide affintiy purification
Form/Format
IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
624
Applicable Applications for anti-KEAP1 antibody
FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human KEAP1 expressed in E. Coli
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.)
WB (Western Blot)
(Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.)
Related Product Information for anti-KEAP1 antibody
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Function: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Function: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 69.7 kDa
Predicted: 70 kDa
Predicted: 70 kDa
NCBI Official Full Name
kelch-like ECH-associated protein 1
NCBI Official Synonym Full Names
kelch like ECH associated protein 1
NCBI Official Symbol
KEAP1
NCBI Official Synonym Symbols
INrf2; KLHL19
NCBI Protein Information
kelch-like ECH-associated protein 1
UniProt Protein Name
Kelch-like ECH-associated protein 1
UniProt Gene Name
KEAP1
UniProt Synonym Gene Names
INRF2; KIAA0132; KLHL19; INrf2
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Product Notes
The KEAP1 keap1 (Catalog #AAA322658) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KEAP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KEAP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KEAP1 keap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KEAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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