LAG-3 recombinant antibody

LAG-3 Single Domain Antibody [2A11]

Cat. Num. AAA40974

• Gene Names: LAG3; CD223
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA
• Purity: LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Synonyms: LAG-3, Recombinant Antibody; LAG-3 Single Domain Antibody [2A11]; LAG3 Antibody: lymphocyte activating 3; LAG-3; CD223; lymphocyte activating 3; LAG-3 recombinant antibody

• Host: Llama
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: 2A11
• Purity/Purification: LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Form/Format: Liquid; LAG-3 Antibody is supplied in PBS.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for LAG-3 recombinant antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Immunogen: LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
• Research Area: Immunology
• NCBI Organism: Homo sapiens
• Preparation and Storage: LAG3 antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5ug/mL.)

FCM/FACS (Flow Cytometry)

(Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1ug/mL.)

ELISA

(Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)

Related Product Information for LAG-3 recombinant antibody

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Product Categories/Family for LAG-3 recombinant antibody

Primary Antibodies

References

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216-21.
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619-22.
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885-91.
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970-9.

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; Q7Z643; A8K7T9
• Molecular Weight: Predicted: 55 kDa; Observed: 54 kDa
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte activating 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN