Mouse anti-Human Lymphocyte Activation Gene 3 (LAG-3) Monoclonal Antibody | anti-LAG-3 antibody

Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)

Cat. Num. AAA215067

• Gene Names: LAG3; CD223
• Reactivity: Human
• Applications: ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Lymphocyte Activation Gene 3 (LAG-3), Antibody; Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator); CD223; FDC protein; LAG-3; anti-LAG-3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: LAG3/3261
• Specificity: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 525

• Applicable Applications for anti-LAG-3 antibody: ELISA
• Positive Control: Human tonsil or Hodgkin's lymphoma (IHC-P).
• Immunogen: Recombinant full-length human LAG3 protein
• Cellular Localization: Membrane.
• Hu-chromosome Location: 12p13.31
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using LAG-3 Mouse Monoclonal Antibody (LAG3/3261). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to LAG-3 (LAG3/3261). Confirmation of Integrity and Purity of Antibody.)

References

1. Andrews LP., et al. Immunol Rev. 2017 Mar;276(1):80-96. doi: 10.1111/imr.12519. 2. Demeure, C.E., et al. 2001. Eur. J. Cancer 37: 1709-171

NCBI and Uniprot Product Information

• NCBI GI #: 251757512
• NCBI GeneID: 3902
• UniProt Accession #: P18627
• Molecular Weight: 70kDa
• NCBI Official Full Name: Lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte activating 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN