WB (Western Blot)
(Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot. Whole cell extract (20 ug) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 1:2000.)
Mouse Lamin A/C Monoclonal Antibody
Lamin A/C antibody (mAb)(Clone 3A6-4C11)
Reactivity
Hamster, Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Culture Supernatant
Synonyms
Lamin A/C, Antibody; Lamin A/C antibody (mAb)(Clone 3A6-4C11); LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; anti-Lamin A/C antibody
Host
Mouse
Reactivity
Hamster, Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3A6-4C11
Purity/Purification
Culture Supernatant
Form/Format
Cell culture supernatant containing mouse IgG, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Lamin A/C antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
Immunogen
This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot. Whole cell extract (20 ug) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 1:2000.)
WB (Western Blot)
(Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot. Whole cell extract (20 ug) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 1:2000.)
IF (Immunofluorescence)
(Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence. Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11). Cells were fixed with 3.7% PFA for 15 minutes at room temperature, permeabilized with PBS + 0.5% Triton X-100 and stained with Lamin A/C mAb (2 ug/ml dilution) followed by 1:500 dilution Chromeo 546 Goat anti-mouse IgG (top panel) and DAPI (bottom panel).)
IF (Immunofluorescence)
(Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence. Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11). Cells were fixed with 3.7% PFA for 15 minutes at room temperature, permeabilized with PBS + 0.5% Triton X-100 and stained with Lamin A/C mAb (2 ug/ml dilution) followed by 1:500 dilution Chromeo 546 Goat anti-mouse IgG (top panel) and DAPI (bottom panel).)
Related Product Information for anti-Lamin A/C antibody
Short Description: Our Lamin A/C antibody (mAb)(Clone 3A6-4C11) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Hamster, Human, Monkey, Mouse and Rat samples.
Background: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.
Background: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.
Product Categories/Family for anti-Lamin A/C antibody
NCBI and Uniprot Product Information
Molecular Weight
75kDa
Similar Products
Product Notes
The Lamin A/C (Catalog #AAA59822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Lamin A/C antibody (mAb)(Clone 3A6-4C11) reacts with Hamster, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin A/C can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Lamin A/C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin A/C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.